Mechanism of Ganoderma lucidum polysaccharides promoting myelin regeneration in demyelinated mice induced by cuprizone
- VernacularTitle:灵孢多糖促进双环己酮草酰二腙诱导的脱髓鞘小鼠髓鞘再生的机制
- Author:
Yan-qing LI
1
;
Xiao-hui LI
;
Qing WANG
;
Li-juan SONG
;
Li-zhi YANG
;
Han-bin WANG
;
Bao-guo XIAO
;
Cun-gen MA
Author Information
- Publication Type:Journal Article
- Keywords: Ganoderma lucidum polysaccharides; cu-prizone; multiple sclerosis; myelin repair and regener-ation; inflammation; JAK2/STAT3 signaling pathway
- From: Chinese Pharmacological Bulletin 2025;41(7):1265-1273
- CountryChina
- Language:Chinese
- Abstract: Aim To explore the mechanism of Gano-derma lucidum polysaccharides(GLPS)promoting my-elin repair and regeneration in mice with chronic demy-elination induced by cuprizone(CPZ).Methods A total of 40 C57BL/6 mice were randomly divided into four groups:Normal+NS,Normal+GLPS,CPZ+NS and CPZ+GLPS.A chronic demyelination model was established using 0.2％CPZ.Open field and elevated plus maze tests were performed to observe the behavior-al changes in the mice.Immunofluorescence staining and Western blot were used to detect changes in myelin basic protein expression in the corpus callosum.ELISA was performed to measure the levels of TNF-α,IL-6,IL-1β and IL-10 in brain homogenates.Immunofluo-rescence staining was also used to observe the expres-sion of ionized calcium binding adapter molecule 1(Iba1)and neural-glial antigen 2(NG2).RT-qPCR and Western blot were conducted to assess the mRNA and protein expression levels of MBP,iNOS,COX-2,JAK2 and STAT3.Results Mice in the CPZ+NS group showed a significant decrease in body weight,cognitive behavior abnormalities,and impaired myelin regeneration.The expression of pro-inflammatory fac-tors increased,while anti-inflammatory factors de-creased.Additionally,Iba1 and NG2 expression in-creased,and the JAK2/STAT3 signaling pathway was activated.After GLPS intervention,the mouse body weight increased,myelin regeneration occurred,cogni-tive behavior was improved,the expression of inflamma-tory factors decreased,anti-inflammatory factors in-creased,NG2 expression was further elevated,and the proliferation of microglia as well as the activation of the JAK2/STAT3 signaling pathway was inhibited.Conclu-sions GLPS can improve cognitive behavior abnormali-ties and inflammatory responses in chronic demyelinated mice by inhibiting the JAK2/STAT3 signaling pathway,thereby promoting myelin repair and regeneration.
