The mechanism of Rutin on multiple organ dysfunction induced by sepsis in mice
10.3969/j.issn.1672-8467.2025.02.009
- VernacularTitle:芦丁对小鼠脓毒症多器官功能障碍的作用与机制研究
- Author:
Zhu-lin YAN
1
;
Fu-peng WU
1
;
Hai-dong LI
1
Author Information
1. 复旦大学附属闵行医院急诊科 上海 201199
- Publication Type:Journal Article
- Keywords:
Rutin;
sepsis;
multiple organ dysfunction;
inflammation;
mice
- From:
Fudan University Journal of Medical Sciences
2025;52(2):232-241
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of Rutin on multiple organ damage in septic mice and to investigate its mechanism from the perspective of inflammation.Methods Male C57BL/6 mice were divided into the normal control(sham)group,cecal ligation and puncture(CLP)group,low-dose Rutin group(25 mg/kg),medium-dose Rutin group(50 mg/kg),and high-dose Rutin group(100 mg/kg),there are 20 mouse in each group.All mice were given gavage daily for 7 days starting at 8 weeks of age(the Rutin groups were administered the corresponding doses of the drug,while the sham and CLP groups were given the same volume of saline).Subsequently,sepsis was induced in mice by CLP.The survival rate of mice was analyzed;pathological damage of the lungs,liver,and kidneys in mice was assessed by HE staining;the lung coefficient and wet/dry(W/D)ratio of the lungs were measured;the levels of alanine transaminase(ALT),aspartate aminotransferase(AST),creatinine(CRE),and urea nitrogen(BUN)in mouse serum were detected;the content of urinary protein in mice was measured;the mRNA levels of tumor necrosis factor-α(TNF-α)and IL-6 in mouse tissues were detected by RT-qPCR;and the activation of the JAK2-STAT3 signaling pathway was analyzed by Western blot.Results Rutin reduced the mortality rate of septic mice,alleviated liver,lung,and kidney damage,improved liver,lung,and kidney functions,inhibited the activation of the JAK2-STAT3 signaling pathway in tissues,and reduced the mRNA expression of pro-inflammatory factors.Conclusion Rutin may alleviate inflammation by inhibiting the activation of the JAK2-STAT3 signaling pathway,and has a protective effect on liver,lung,and kidney damage in septic mice.
