A method for preparing efficient adipogenic differentiation of mice adipose stem cells
10.3969/j.issn.1672-8467.2025.02.015
- VernacularTitle:一种高效成脂分化的小鼠脂肪干细胞的制备方法
- Author:
Qiu-kai TANG
1
;
Sai-sai SONG
;
Yan TANG
Author Information
1. 浙江新安国际医院检验科 嘉兴 314031
- Publication Type:Journal Article
- Keywords:
adipose stem cell;
adipocyte;
differentiation;
stromal vascular fraction
- From:
Fudan University Journal of Medical Sciences
2025;52(2):277-284,291
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a simple method for producing mice adipose stem cells with highly efficient adipogenic differentiation capability.Methods Subcutaneous adipose tissue was extracted from wild-type mice.By strictly controlling the digestion time of collagenase,we compared the number of adipose stem cells and their adipogenic differentiation ability obtained for different digestion times.Further,flow cytometry was used to clarify the differences in mesenchymal cells and vascular components at different digestion times.Results The adipose stem cells obtained after 40 minutes of digestion by collagenase exhibited over 90%adipogenic differentiation ability in vitro,while those obtained after 60 minutes of digestion only showed 20%adipogenic differentiation ability.The main reason for this discrepancy was the presence of a portion of vascular-related cells in the latter.Conclusion By controlling the digestion time of collagenase in adipose tissue,we have established an efficient method for preparing adipose stem cells with high adipogenic differentiation potential.The obtained adipose stem cells exhibit over 90%adipogenic differentiation ability in vitro.
