Glimepiride alleviates cerebral edema and apoptosis in rats with subarachnoid hemorrhage by inhibiting SUR1-TRPM4 channel
10.16557/j.cnki.1000-7547.2025.01.010
- VernacularTitle:格列美脲通过抑制SUR1-TRPM4通道减轻蛛网膜下腔出血大鼠脑水肿和凋亡
- Author:
Guangzhi HAO
1
;
Yujia DONG
;
Yu HUAN
;
Yushu DONG
Author Information
1. 中国人民解放军北部战区总医院神经外科,沈阳 110016
- Publication Type:Journal Article
- Keywords:
glimepiride;
sulfonylurea receptor 1(SUR1);
transient receptor potential melastatin 4(TRPM4);
subarachnoid hemorrhage(SAH);
brain edema;
early brain injury(EBI);
rat
- From:
Chinese Journal of Neuroanatomy
2025;41(1):67-72
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of glimepiride on cerebral edema and apoptosis in a rat model of sub-arachnoid hemorrhage(SAH).Methods:An SAH model was established in rats using the internal carotid artery punc-ture method.Low-dose glimepiride was administered via intraperitoneal injection.Neurological deficits were assessed u-sing the modified Neurological Severity Score(mNSS),and brain water content was evaluated by measuring the wet-dry weight ratio of brain tissue.Cortical tissue from the surgical side was collected to measure the mRNA expression of sul-fonylurea receptor 1(SUR1)and transient receptor potential melastatin 4(TRPM4)using RT-qPCR,while the protein expression of Bcl-2 and Bax was detected by Western blot.Results:SAH rats exhibited impaired neurological function,increased brain water content,upregulation of SUR1 and TRPM4 mRNA expression,and a decreased Bcl-2/Bax ratio.Low-dose glimepiride did not affect blood glucose levels but significantly improved neurological function and reduced cerebral edema in SAH rats.Although glimepiride had no significant effect on SUR1 and TRPM4 mRNA expression,it increased the Bcl-2/Bax ratio.Conclusion:Glimepiride alleviates cerebral edema and inhibits apoptosis in SAH model rats by suppressing the SUR1-TRPM4 channel.
