Comparison of the actions of different anesthetics in the post-stroke dysphagia model of mouse
10.16557/j.cnki.1000-7547.2025.01.009
- VernacularTitle:脑卒中后吞咽障碍小鼠模型中不同麻醉剂作用的比较
- Author:
Tong RAO
1
;
Zehua RAO
1
;
Cong TIAN
1
;
Meng LU
1
;
Ankun CHEN
1
;
Xin LIU
1
;
Zhimiao MURONG
1
;
Zenghui YUE
1
Author Information
1. 湖南中医药大学针灸推拿与康复学院,长沙 410208
- Publication Type:Journal Article
- Keywords:
isoflurane;
pentobarbital sodium;
tribromoethanol;
photothrombosis;
post-stroke dysphagia(PSD);
swallowing function;
mouse
- From:
Chinese Journal of Neuroanatomy
2025;41(1):59-66
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects three of anesthetics(tribromoethanol,isoflurane,and pentobarbital so-dium)on the outcome of mice with post-stroke dysphagia(PSD)induced by photothrombosis(PT)method,and to e-valuate which anesthetic is more suitable for the preparation of this model.Methods:Sixty-six male C57BL/6J mice were divided into Tribromoethanol group,Isoflurane group,Pentobarbital sodium group and Sham group.The post-stroke dysphagia model was established by PT.Before and 5 min after modeling,a laser speckle imager was used to measure the regional cerebral blood flow(rCBF)decrease rate of mice and record the wake-up time of mice.Forty-eight hours after modeling,the mortality rate of PSD mice in three groups was recorded and the rCBF decrease rate was meas-ured again.The neurological function of mice was evaluated using the neurological deficit score,the water intake of mice was recorded using the 4-min drinking test,the infarct volume ratio was measured using the TTC staining method,and the swallowing counts induced by water administration was recorded using the multichannel physiological recorder MP160 and the myoelectric area of the swallowing muscle was calculated.Results:There was no statistical difference in the percentage of decrease in rCBF,infarct volume ratio,neurological deficit score,water intake,swallowing counts,and myoelectric area of swallowing muscle among the three groups of PSD mice 48 h after modeling(P＞0.05).Com-pared with the Tribromoethanol group and the Pentobarbital sodium group,the rCBF of the mice in the Isoflurane group decreased rapidly within 5 min(P＜0.05),and the mortality rate of the mice was lower and the awakening time was shorter.(P＜0.05).Conclusion:The use of different anesthesia will affect the mortality rate,wake-up time and the downward trend of rCBF within 48 h after modeling of PSD mice.Among the three anesthetics,isoflurane is more suit-able as an anesthetic for the PSD mouse model.
