Analgesic effect of fire needle on rats with postherpetic neuralgia and its effect on IL-10/β-EP signaling pathway
10.3969/j.issn.1000-484X.2025.03.009
- VernacularTitle:火针对带状疱疹后神经痛大鼠的镇痛作用及其对IL-10/β-EP信号通路的影响
- Author:
Han LI
1
;
Hongfei ZHOU
;
Jun LIU
;
Wei GU
Author Information
1. 辽宁中医药大学,沈阳 110847
- Publication Type:Journal Article
- Keywords:
Postherpetic neuralgia;
Fire needle;
Analgesia;
IL-10;
β-endorphin;
Inflammation
- From:
Chinese Journal of Immunology
2025;41(3):561-565
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate analgesic effect of fire needle on postherpetic neuralgia(PHN)rats and its effect on IL-10/β-endorphin(β-EP)signaling pathway.Methods:Forty rats were randomly divided into 4 groups:control group,model group,fire needle group and drug group,with 10 rats in each group.PHN model was constructed by intraperitoneal injection of resin toxin.Af-ter successful modeling,fire needle group was intervened by fire needle at L4～6 Jiaji points,and drug group was intervened by intra-peritoneal injection of gabapentin.Von Frey electronic pain meter was used to measure paw withdrawal mechanical threshold(PW-MT)of rats in each group.Thermal latency of paw withdrawal(PWTL)of rats was measured by thermal radiation stimulator.Prosta-glandin E2(PGE2)and substance P(SP)in dorsal root ganglion tissues,serum IL-6,TNF-α and IL-1β levels were detected by ELI-SA.IL-10 and β-EP protein expressions in spinal cord of rats were detected by Western blot.Results:Compared with control group,PWMT of model group was decreased significantly(P<0.05),PWTL,levels of PGE2 and SP in spinal cord tissue and levels of IL-6,TNF-α and IL-1β in serum were increased significantly(P<0.05),IL-10 and β-EP protein expressions in spinal dorsal horn were decreased significantly(P<0.05).Compared with model group,PWMT of fire needle group and drug group was significantly increased(P<0.05),PWTL,PGE2 and SP in spinal cord tissue and serum IL-6,TNF-α and IL-1β levels were significantly decreased(P<0.05),IL-10 and β-EP protein expressions in spinal dorsal horn were significantly increased(P<0.05).Conclusion:Fire needle can improve pain response of PHN rats and reduce peripheral blood inflammation level,which may be related to activation of IL-10/β-EP signaling pathway.
