Impact of atractylolide Ⅰ on liver function in hepatitis B model rats by regulating cGAS-STING pathway
10.3969/j.issn.1000-484X.2025.03.018
- VernacularTitle:白术内酯Ⅰ调节cGAS-STING通路对乙型肝炎模型大鼠肝功能的影响
- Author:
Chengmei HAN
1
;
Maoli CHEN
1
;
Weixin REN
1
Author Information
1. 山东第一医科大学附属人民医院感染科,济南 271100
- Publication Type:Journal Article
- Keywords:
Atractylolide Ⅰ;
cGAS-STING pathway;
Hepatitis B virus;
Liver fibrosis;
Liver function
- From:
Chinese Journal of Immunology
2025;41(3):615-619
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate impact of atractylolidⅠ(ATR-Ⅰ)on liver function of hepatitis B virus(HBV)infection rats by regulating cGAS-STING pathway.Methods:Ten rats were randomly selected as control group(NC),rest rats were injected with HBV antigen via tail vein to construct HBV model.Rats successfully modeled were randomly grouped into HBV group,ATR-Ⅰgroup(50 μg/kg),inhibitor of cGAS-STING pathway(RU.521)group(450 μg/kg),ATR-Ⅰ+RU.521 group(50 μg/kg ATR-Ⅰ+450 μg/kg RU.521),with 10 rats in each group.NC group and HBV group were injected with same amount of normal saline once a day for 1 week.Serum aspartate aminotransferase(AST),alanine aminotransferase(ALT),hyaluronic acid(HA),laminin(LN),procolla-gen type Ⅲ(PC Ⅲ),IFN-γ,IL-2,TNF-α,hepatitis B surface antigen(HBsAg),hepatitis B e antigen(HBeAg)were detected by ELISA;HE staining was applied to detect liver histopathology;Masson staining was applied to detect degree of liver fibrosis;Western blot was applied to detect expressions of cGAS-STING pathway proteins in liver tissue.Results:Collagen volume fraction,ALT,AST,HBsAg,HBeAg contents,HA,LN and PC Ⅲ,IFN-γ,TNF-α and IL-2 levels in HBV group were higher than NC group,levels of cGAS,STING,p-TBK1/TBK1,p-IRF3/IRF3 and IFN-β were decreased(P<0.05);compared with HBV group,degree of liver injury and liver fibrosis in ATR-Ⅰ group were improved,collagen volume fraction,ALT,AST,HBsAg,HBeAg contents,HA,LN,PC Ⅲ,IFN-γ,TNF-α and IL-2 levels were decreased,levels of cGAS,STING,p-TBK1/TBK1,p-IRF3/IRF3 and IFN-β were in-creased(P<0.05),RU.521 group showed opposite trend,RU.521 eliminated improvement effect of ATR-Ⅰ on liver function of HBV rats.Conclusion:ATR-Ⅰmay improve liver function of HBV rats by activating cGAS-STING pathway.
