Effect of inhibiting of miR-203a-3p gene expression on proliferation and apoptosis of rheumatoid arthritis synovial fibroblasts
10.3969/j.issn.1000-484X.2025.03.016
- VernacularTitle:抑制miR-203a-3p基因表达对类风湿关节炎滑膜成纤维细胞增殖与凋亡的影响
- Author:
Lirong CHEN
1
;
Qiuyu FAN
1
;
Ya LIU
1
;
Huiqin YANG
1
Author Information
1. 武汉市第一医院风湿免疫科,武汉 430022
- Publication Type:Journal Article
- Keywords:
miR-203a-3p;
Rheumatoid arthritis;
Proliferation;
Apoptosis
- From:
Chinese Journal of Immunology
2025;41(3):600-604
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of inhibiting miR-203a-3p gene expression on proliferation and apoptosis of rheu-matoid arthritis(RA)synovial fibroblasts.Methods:Divided fibroblast-like synovial cells MH7A into Control group(untransfected cells),anti-miR-NC group(transfected with anti-miR-NC),anti-miR-203a-3p group(transfected with anti-miR-203a-3p),anti-miR-203a-3p+LiCl group(transfected with anti-miR-203a-3p+signal pathway activator LiCl).qRT-PCR was used to detect expression level of miR-203a-3p;clone formation experiment,MTT experiment were used to detect cell proliferation;flow cytometry was used to de-tect cell apoptosis;Western blot was used to detect protein expressions of PCNA,Bcl-2,Bax,Wnt1,β-catenin.Results:Transfec-tion of miR-203a-3p reduced the number of clones and survival rate of MH7A cells,increased rate of apoptosis,reduced expression levels of PCNA,Bcl-2,Wnt1,β-catenin protein,increased expression level of Bax protein.The signaling pathway activator LiCl in-creases the number of clones and survival rate of MH7A cells transfected with miR-203a-3p,reduces the rate of apoptosis,increases the expression level of Wnt1,β-catenin,PCNA,Bcl-2 protein,and reduces expression level of Bax protein.Conclusion:miR-203a-3p may promote proliferation of RA synovial fibroblasts and inhibit cell apoptosis by activating Wnt/β-catenin.
