Effects of lncRNA NEAT1 targeting miR-495-3p on WI-38 cell injury induced by LPS
10.3969/j.issn.1000-484X.2025.03.012
- VernacularTitle:lncRNA NEAT1靶向miR-495-3p对LPS诱导的WI-38细胞损伤的影响
- Author:
Xi ZHU
1
;
Yun GUO
;
Tao LU
;
Jun QIAN
Author Information
1. 江南大学附属儿童医院塈无锡市儿童医院重症医学科,无锡 214023
- Publication Type:Journal Article
- Keywords:
lncRNA NEAT1;
miR-495-3p;
Human embryo lung cell WI-38;
Apoptosis;
Inflammation
- From:
Chinese Journal of Immunology
2025;41(3):576-581
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of long non-coding RNA(lncRNA)nuclear enriched transcript 1(NEAT1)on lipopolysaccharide(LPS)-induced human embryonic lung cells(WI-38)injury by regulating microRNA-495-3p(miR-495-3p).Methods:WI-38 cells were treated with 10 μg/ml LPS to establish an in vitro pneumonia model.Real-time fluorescent quantitative polymerase chain reaction(qPCR)was used to detect the expression levels of NEAT1 and miR-495-3p in WI-38 cells.The dual lucif-erase reporter assay was used to detect the targeting relationship between NEAT1 and miR-495-3p.Cell counting kit and flow cytome-try were used to detect the effect of knockdown NEAT1 on the proliferation and apoptosis of WI-38 cells treated with LPS.Western blot was used to detect Bcl-2,Bax and Cleaved caspase-3 protein expressions.ELISA was used to detect the levels of inflammatory factors IL-6,IL-8 and IL-1β.Results:LPS stimulation could up-regulate the expression of NEAT1 and inhibit the expression of miR-495-3p in WI-38;NEAT1 directly targeted miR-495-3p,and NEAT1 knockdown could promote the expressions of miR-495-3p.Functionally,LPS inhibited the proliferation of WI-38 cells and promoted cell apoptosis and the expression of the inflammatory factors IL-6,IL-8 and IL-1β(P<0.05).Knockdown of NEAT1 could inhibit LPS-induced WI-38 cell apoptosis and inflammation,and promote cell pro-liferation.Conclusion:Knockdown of NEAT1 can inhibit LPS-induced apoptosis and inflammation of WI-38 cells by negatively regu-lating miR-495-3p.
