Matrine inhibits proliferation of human umbilical vein endothelial cells by regulating miR-125b-5p/STAT3 pathway
10.3969/j.issn.1000-484X.2025.03.008
- VernacularTitle:苦参碱通过调控miR-125b-5p/STAT3途径抑制人脐静脉内皮细胞增殖
- Author:
Xing WANG
1
;
Huiming ZHU
;
Huan MA
;
Chao WANG
;
Huixin ZHANG
Author Information
1. 河北省人民医院代谢病重点实验室,石家庄 050051
- Publication Type:Journal Article
- Keywords:
Matrine;
Human umbilical vein endothelial cells;
Proliferation;
miR-125b-5p;
STAT3
- From:
Chinese Journal of Immunology
2025;41(3):556-560
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study effect of matrine on TNF-α induced proliferation of human umbilical vein endothelial cells(HUVEC),as well as to explore whether its mechanism is related to regulation of miR-125b-5p and STAT3 expressions.Methods:HUVEC proliferation model was established by TNF-α stimulating.After matrine treatment,miR-125b-5p level was detected by real-time fluorescent quantitative PCR,proliferating cell nuclear antigen(PCNA),cell cycle protein D1(CyclinD1),matrix metallopro-teinase 2(MMP2),MMP9,STAT3 levels were detected by real-time fluorescent quantitative PCR and Western blot.Targeting rela-tionship between miR-125b-5p and STAT3 was verified by dual luciferase reporter gene assay.Results:Matrine inhibited TNF-α in-duced HUVEC proliferation(P<0.05),decreased PCNA,CyclinD1,MMP2,MMP9,STAT3 expressions(P<0.05),and increased miR-125b-5p expression(P<0.05).Overexpression of miR-125b-5p could reduce cell proliferation and PCNA,CyclinD1,MMP2,MMP9 expressions HUVEC induced by TNF-α(P<0.05).miR-125b-5p targeted STAT3 expression negatively in HUVEC,and inhi-biting miR-125b-5p expression could reverse effect of matrine on TNF-α induced cell proliferation and PCNA,CyclinD1,MMP2,MMP9 and STAT3 expressions.Conclusion:Matrine inhibits TNF-α induced proliferation of HUVEC,which is related to regulation of miR-125b-5p/STAT3 pathway.
