Protective effect of catalpol on liver injury in acute hepatitis B rats by regulating TLR4/NF-κB signaling pathway
10.3969/j.issn.1000-484X.2025.03.006
- VernacularTitle:梓醇调节TLR4/NF-κB信号通路对急性乙型肝炎大鼠肝损伤的保护作用研究
- Author:
Xuexia LIAO
1
;
Xin PENG
1
;
Tingting HUANG
1
;
Guidan XIE
1
Author Information
1. 儋州市人民医院消化内科,儋州 571799
- Publication Type:Journal Article
- Keywords:
Hepatitis B;
Catalpol;
Immune response;
Inflammatory response
- From:
Chinese Journal of Immunology
2025;41(3):545-550
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the protective effect of catalpol on the liver of rats with acute hepatitis caused by hepatitis B virus(HBV)infection and its possible mechanism.Methods:SD rats were randomly divided into Sham group,Model group,acyclo-vir group(ACY group),low-dose catalpol group(L-CAT group),medium-dose catalpol group(M-CAT group)and high-dose catalpol group(H-CAT group).The levels of serum inflammatory cytokines were detected by ELISA.Flow cytometry was used to detect the per-centage of peripheral blood Th17 cells.Hematoxylin-eosin staining and TUNEL assay were used to detect liver tissue injury and apopto-sis.Western blot analysis was used to detect the expression of related proteins in liver tissue.Results:Compared with the Sham group,the levels of serum HBsAg,HBeAg,ALT,AST,Th17 cell ratio,IL-17 and IL-23,and the expression of TLR4 and p-p65 protein in liver tissues were significantly increased in the Model group,while the levels of serum IL-13 and IL-4 were significantly decreased in the Model group,the differences were statistically significant(P<0.05).Compared with the Model group,the levels of serum HBsAg,HBeAg,ALT,AST,Th17 cell ratio,IL-17 and IL-23,as well as the expressions of TLR4 and p-p65 protein in liver tissues of rats in the ACY,M-CAT and H-CAT groups were significantly decreased,while the levels of serum IL-13 and IL-4 were significantly in-creased,the differences were statistically significant(P<0.05).Compared with the L-CAT and M-CAT groups,the levels of serum HB-sAg,HBeAg,ALT,AST,Th17 cell ratio,IL-17 and IL-23,the expression of liver TLR4 and p-p65 protein were significantly de-creased,while the levels of serum IL-13 and IL-4 were significantly increased in the H-CAT group,the differences were statistically significant(P<0.05).Conclusion:Catalpol improves HBV infection-induced liver injury in rats by reducing Th17 cell proportion and pro-inflammatory cytokine levels,and increasing anti-inflammatory cytokine levels,possibly by inhibiting TLR4/NF-κB p65 signaling pathway activation.
