Mechanism of resveratrol attenuating brain injury after ischemic stroke via NOX2-mediated polarization of microglia
10.3969/j.issn.1000-484X.2025.03.024
- VernacularTitle:白藜芦醇通过NOX2介导的小胶质细胞极化减轻脑卒中后脑损伤的机制研究
- Author:
Xiaoqun HE
1
;
Chunhua LUO
;
Ling HUANG
;
Gang XIAO
Author Information
1. 右江民族医学院附属医院药学部,百色 533000
- Publication Type:Journal Article
- Keywords:
Resveratrol;
Ischemic stroke;
Nico-tinamide adenine dinucleotide phosphate oxidase 2;
Microglial polarization
- From:
Chinese Journal of Immunology
2025;41(3):650-654
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate protective mechanism of resveratrol(RES)on brain injury after ischemic stroke(IS)and its regulatory mechanism on nico-tinamide adenine dinucleotide phosphate oxidase 2(NOX2)mediated polarization of microglia.Methods:Low and high doses of RES were used for intervention.Tetrabromocinnamic acid(TBCA),a specific agonist of NOX2,was used to save function.PC12 and LMAI Bio cell models with oxygen-glucose deprivation/reoxygenation(OGD/R)were constructed.TUNEL was used to detect apoptosis of PC12 cells.Immunofluorescence was used to detect polarization of LMAI Bio cells.Western blot was used to detect expressions of NOX2,NF-κB,TNF-α,IL-1β,IL-10 and TGF-β in PC12 and LMAI Bio cells.Results:RES could significantly inhibit apoptosis of PC12 cells induced by OGD/R,and regulate LMAI Bio cells polarization to M2 type.Western blot showed that RES could significantly down-regulate NOX2,NF-κB,TNF-α and IL-1β expressions in OGD/R cells,up-regulate IL-10 and TGF-β expressions,while TBCA could partially reverse protective effect of RES on neurons.Conclusion:RES can inhibit neuronal apoptosis after IS,which may be related to its regulation of NOX2-mediated polarization of microglia.
