SNG162 activates the estrogen signaling pathway at the membrane initiation of glioblastoma by upregulating ER-α36 ex-pression
10.11904/j.issn.1002-3070.2025.03.002
- VernacularTitle:SNG162通过上调ER-α36表达激活胶质母细胞瘤膜起始的雌激素信号通路
- Author:
Entong ZHAO
1
;
Xin GUAN
;
Hongyan LI
;
Yunting QIU
;
Bingqiang ZHANG
;
Mengmeng CHEN
;
Wei ZOU
;
Chao QU
Author Information
1. 辽宁师范大学生命科学学院(大连 116081);辽宁师范大学生物技术与分子生物学药物研发重点实验室
- Publication Type:Journal Article
- Keywords:
Glioblastomas;
Estrogen receptor-alpha36;
Estrogen;
Icariin isomer
- From:
Practical Oncology Journal
2025;(3):177-183
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study was to explore the effects of changes in the estrogen receptor alpha 36(ER-α36)expression on the proliferation and membrane-initiated estrogen signaling in glioblastoma U251 cells.Methods The expression and localization of ER-α36 and EGFR glioblastoma U87 cells and U251 cells were determined by immunofluorescence,qRT-PCR and Western blot.The effect of upregulating ER-α 36 on U251 cell proliferation and estrogen signaling pathway activity by low con-centrations of 100 pmol/L icariin isomer(SNG162)was detected by MTT assay and Western blot.Results ER-α36 and EGFR were co-expressed in the cell membrane of glioblastoma.Compared with DMSO(control group),the expression ER-α36(P<0.01)and EG-FR increased in U251 cells treated with SNG162(P<0.05);Further experments also found that low concentrations of SNG162 in-creased the expression of cycle related proteins-cyclin D1,cyclin B,cyclin E and CDK4(P<0.01),and enhanced the proliferative a-bility of U251 cells(P<0.05).Conclusion The low concentration of SNG162 upregulates the expression of ER-α36,activates the estrogen-mediated ERK1/2 MAPK,p38 MAPK,and EGFR/Src signaling pathways,promotes glioblastoma proliferation,and activates the membrane initialized estrogen signaling pathway.
