Exploring the Mechanism of Huatan Qushi Huoxue Recipe in the Treatment of NASH Based on Network Pharmacology and Animal Experi-ments
10.11969/j.issn.1673-548X.2025.05.011
- VernacularTitle:基于网络药理学和动物实验探究化痰祛湿活血方治疗非酒精性脂肪性肝炎的作用机制
- Author:
Yajie GUAN
1
;
Lihui ZHANG
;
Sutong LIU
Author Information
1. 450000 郑州,河南中医药大学第一附属医院脾胃肝胆科;450046 郑州,河南中医药大学第一临床医学院
- Publication Type:Journal Article
- Keywords:
Huatan Qushi Huoxue recipe;
NASH;
RAGE;
AP-1
- From:
Journal of Medical Research
2025;54(5):52-59,51
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the potential mechanisms of action of Huatan Qushi Huoxue Recipe in treating non-alcoholic steatohepatitis(NASH)through network pharmacology and animal experiments.Methods The differentially expressed genes in NASH were obtained from the GEO database.The active components and potential targets of Huatan Qushi Huoxue Recipe were obtained from the TCMSP.The treatment targets were obtained by intersecting the diseases and drug targets.The protein-protein interaction network was analyzed,the drug-active component-target network was constructed,and the enrichment analysis was performed.The key pathways were verified by animal experiments.Results A total of 74differentially expressed genes,97 active components,and 295 potential targets were identified in NASH.Five genes,including JUN,were selected as key targets through intersection.Seven active components,inclu-ding kaempferol and quercetin,were identified.Enrichment analysis revealed that the AGE-RAGE and IL-17 pathways may play a key role in the treatment of NASH by Huatan Qushi Huoxue Recipe.Animal experiments showed that Huatan Qushi Huoxue Recipe could reduce the levels of total cholesterol(TC),triglyceride(TG),and blood glucose(GLU),and down-regulate the expression of RAGE and activator protein 1(AP-1)in the AGE-RAGE and IL-17 pathways,thus,exerting a therapeutic effect on NASH.Conclusion Huatan Qushi Huoxue Recipe can treat NASH by lowering TC,TG and GLU levels and inhibiting the expression of RAGE and AP-1 pro-tein.
