Expression of miR-30c-5p and CHI3L1 in colorectal cancer tissue and their relationship with clinical pathological features and lymph node metastasis
10.3969/j.issn.1005-6483.20240806
- VernacularTitle:结直肠癌组织微小核糖核酸-30c-5p、非酶几丁质酶-3样蛋白-1表达及与其临床病理特征、淋巴结转移的关系
- Author:
Yong LI
1
;
Hongbo WANG
;
Lei LI
Author Information
1. 233000 安徽蚌埠,蚌埠医科大学第一附属医院普外科
- Publication Type:Journal Article
- Keywords:
colorectal cancer;
microribonucleic acid-30c-5p;
chitinase-3-like protein 1;
clinical pathological features;
lymph node metastasis
- From:
Journal of Clinical Surgery
2025;33(6):628-631
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate and analyze the expression of microribonucleic acid-30c-5p(miR-30c-5p)and chitinase-3-like protein 1(CHI3L1)in colorectal cancer tissue,and their relationship with clinical pathological features and lymph node metastasis.Methods 106 colon cancer patients admitted to First Affiliated Hospital of Bengbu Medical College from January 2022 to July 2024 were gathered.The patients were divided into two groups based on whether lymph node metastasis occurred.There were 50 cases in the metastasis group and 56 cases in the non-metastasis group.The cancer tissues and adjacent tissues were collectedQRT-PCR and immunohistochemistry were applied to detect the expression of miR-30c-5p and CHI3L1 in cancer and adjacent tissues.Multivariate Logistic regression was applied to analyze the influencing factors of lymph node metastasis in patients.Results Compared with adjacent tissues,the expression level of miR-30c-5p(0.74±0.08 vs 1.00±0.11)in cancer tissues was obviously reduced(P＜0.05),and the positive expression rate of CHI3L1(67.92％vs 39.62％)was obviously increased(P＜0.05).There were statistically significant differences in the expression of miR-30c-5p and CHI3L1 in cancer tissues of patients with different tumor diameters(68.42％vs 44.90％,65.28％vs 41.18％),TNM staging(40.35％vs 63.27％,67.65％vs 43.06％),tissue differentiation degree(36.84％vs 59.18％,38.89％vs 64.71％),and lymph node metastasis(49.12％vs 24.49％,29.41％vs 55.56％)(P＜0.05).There were statistically significant differences in tumor diameter,tissue differentiation,TNM staging,vascular invasion,neurological invasion and expression of miR-30c-5p(0.85±0.09 vs 0.62±0.07),CHI3L1(57.14％vs 80.00％)and carcinoembryonic antigen(CEA)(3.25±0.38 vs 4.21±0.45)between the metastasis group and non metastasis group(P＜0.05).The Logistic regression analysis showed,the Elevated expression of miR-30c-5p[OR(95％CI)=0.682(0.500-0.930)]was a protective factor affecting lymph node metastasis in patients(P＜0.05),while positive expression of CHI3 L1,low tissue differentiation,TNM staging T3+T4 and the level of CEA[OR(95％CI)=1.593(1.037-2.447),1.852(1.155-2.970),1.603(0.926-2.775),1.742(1.006-3.016)]were risk factors affecting lymph node metastasis in patients(P＜0.05).Conclusion The expression level of miR-30c-5p is obviously reduced in cancer tissues of colon cancer patients,while CHI3L1 is positively expressed and they are associated with clinicopathological features,both of which are influential factors in the development of lymph node metastasis in patients.
