In vivo study on effects of Sox30 gene point mutation on spermatogenesis and sperm motility in male mice
10.13602/j.cnki.jcls.2025.06.10
- VernacularTitle:Sox30基因点突变对雄性小鼠生精过程以及精子活力影响的体内研究
- Author:
Niuniu CHEN
1
;
Sen REN
1
;
Lan YE
1
Author Information
1. 南京医科大学生殖医学与子代健康全国重点实验室,南京 211166
- Publication Type:Journal Article
- Keywords:
Sox30;
non-obstructive azoospermia;
gene mutation;
mice;
testis
- From:
Chinese Journal of Clinical Laboratory Science
2025;43(6):444-449
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Sox30 gene mutations on spermatogenesis and sperm functionality.Methods CRISPR-Cas9 technology was used for gene editing to generate Sox30P636S mutant mice,and their genotypes were confirmed by Sanger sequencing.Sperm count,computer-assisted sperm analysis,hematoxylin-eosin(H&E)staining,and immunofluorescence were em-ployed to investigate the effects of Sox30 mutation on spermatogenesis and sperm viability in the mice.Results Compared to wild-type Sox30+/+mice,heterozygous Sox30P636S/+and homozygous Sox30P636S/P636S mice did not show statistically significant differences in testicu-lar size,weight,or mature sperm count(P＞0.5).Sperm motility analysis revealed that the proportion of motile sperm in homozygous Sox30P636S/P636S mice was approximately 10％ lower than that in wild-type Sox30+/+mice(P＜0.05),but no obvious abnormalities were observed in sperm formation process of the homozygous mice.Conclusion Although the Sox30P636S point mutation did not significantly impact the spermatogenesis process in mice,it moderately impaired their sperm motility to some extent.
