Expression and significance of B4GALT1 and PD-L1 in lung adenocarcinoma and lung squamous cell carcinoma
10.13315/j.cnki.cjcep.2025.06.007
- VernacularTitle:肺腺癌与肺鳞状细胞癌中B4GALT1、PD-L1的表达及意义
- Author:
Lan GAO
1
;
Chenggang YANG
1
Author Information
1. 云南省肿瘤医院/北京大学肿瘤医院云南医院/昆明医科大学第三附属医院病理科,昆明 650100
- Publication Type:Journal Article
- Keywords:
pulmonary neoplasms;
lung adenocarcinoma;
lung squamous cell carcinoma;
PD-L1;
B4GALT1;
immu-nohistochemistry
- From:
Chinese Journal of Clinical and Experimental Pathology
2025;41(6):739-745
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the correlation between B4GALT1 and PD-L1 expression in lung adenocarci-noma and lung squamous cell carcinoma,and to assess their clinicopathological significance.Methods The EnVision immunohistochemical method was used to detect B4GALT1 and PD-L1 protein expression in 195 cases of lung adeno-carcinoma,105 cases of lung squamous cell carcinoma,and 300 cases of non-cancer lung tissue.The relationships be-tween these protein expression levels and the clinicopathological features of both tumor types,as well as the correlation between B4GALT1 and PD-L1 expression were analyzed.Additionally,bioinformatics approaches were employed to ex-amine the correlation of B4GALT1 and PD-L1 gene expression and to evaluate their impact on patient prognosis in both cancer subtypes.Results The positive rate of B4GALT1 protein in lung adenocarcinoma(66.7％,130/195)was significantly higher than that in lung squamous cell carcinoma(25.7％,27/105),whereas PD-L1 positivity was grea-ter in squamous cell carcinoma(63.8％,67/105)than that in adenocarcinoma(37.9％,74/195).The difference betweenthe two groups was statistically significant(P＜0.05).Both proteins were undetectable in non-cancer lung tis-sues.In lung adenocarcinoma,the expression of both B4GALT1 and PD-L1 was significantly correlated with TNM stage,maximum tumor diameter,pathological subtype,and lymph node metastasis(P＜0.05);And PD-L1 expression was also correlated with distant metastasis(P＜0.05).In lung squamous cell carcinoma,B4GALT1 expression showed no significant association with sex,age,TNM stage,maximum tumor diameter,differentiation,lymph node metastasis,distant metastasis and smoking history(P＞0.05),while PD-L1 expression correlated with TNM stage(P＜0.05).A positive correlation between B4GALT1 and PD-L1 protein expression was observed in adenocarcinoma(r=0.438,P＜0.05).Bioinformatics analysis confirmed a positive correlation at the gene-expression level(r=0.32,P＜0.05).Ad-enocarcinoma patients exhibiting high B4GALT1 and PD-L1 expression experienced lower overall survival(P＜0.05),while in lung squamous cell carcinoma,high PD-L1 expression was associated with prolonged overall survival(P＜0.05).Conclusion B4GALT1 and PD-L1 are positively correlated in lung adenocarcinoma and were significantly in-volved in its tumorigenesis,progression,invasiveness,and clinical prognosis.B4GALT1 may represent a promising therapeutic target in future lung cancer treatment.
