Feasibility Exploration of Machine-based Platelet Washing and In Vitro Quality Report
10.3870/j.issn.1672-0741.24.09.004
- VernacularTitle:机制洗涤血小板的可行性探讨和体外质量报告
- Author:
Xue ZHENG
1
;
Lihua DAI
1
;
Mengdi MA
1
Author Information
1. 武汉血液中心成分制备科,武汉 430030
- Publication Type:Journal Article
- Keywords:
platelet washing;
standardization;
in vitro quality;
ACP215
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2025;54(3):395-400
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare washed platelets by automatic blood cell treatment system ACP215 and evaluate the in vitro quality of the platelets after washing.Methods Based on the ACP215 washing program,the improved disposable consum-ables,as well as the prepared platelet washing solution and suspension,were applied to automatically wash and prepare single bags of platelets.The platelet volume,platelet content,plasma protein content,PDW,MPV and PH before and after washing were compared,and compared with the traditional manual washing method.After storage for 24 hours,platelet aggregation test,thromboelastography MA evaluation and subcellular structure photoimaging were performed.Results The recovery rate was(87.55±2.54)％and plasma protein clearance was(95.84±0.69)％,which were significantly higher than the conventional manual method(Z=-13.633,P＜0.01;Z=-12.039,P＜0.01).There was no statistical difference in maximum aggregation and aggregation time of platelets before and after washing(t=1.273,P=0.218;t=0.770,P=0.451).MA value was slightly reduced(t=2.236,P=0.038).There was no statistical difference in subcellular structure before and after washing(x2=2.257,P=0.133;x2=0.523,P=0.469).Conclusion Utilization of automatic blood cell treatment system ACP215 can standardize and automatically prepare washing platelets which meet the quality requirements.The platelets has good in vitro function after washing and 24 hours of storage.
