Periodic expression of ERα and ClC-3 and their correlation with anti-breast cancer effect of tamoxifen
10.3969/j.issn.1000-4718.2025.03.001
- VernacularTitle:ERα和ClC-3的周期性表达与他莫昔芬抗乳腺癌作用的相关性研究
- Author:
Xueke LI
1
;
Xiuying HOU
;
Shiqing LIU
;
Haifeng YANG
;
Linyan ZHU
;
Weili HE
Author Information
1. 暨南大学基础医学与公共卫生学院药理学系,广东 广州 510630
- Publication Type:Journal Article
- Keywords:
breast cancer;
estrogen receptor α;
ClC-3 chloride channel;
tamoxifen;
cell cycle
- From:
Chinese Journal of Pathophysiology
2025;41(3):417-426
- CountryChina
- Language:Chinese
-
Abstract:
AIM:This study aims to investigate of perodic expression,distribution and interaction between es-trogen receptor α(ERα)and ClC-3 chloride channel,and their relevance to the cell cycle specificity of tamoxifen(TAM)in anti-breast cancer treatment.METHODS:We utilized a web database to analyze the correlation between ERα and ClC-3 expression.Three-dimentional molecular simulation software and co-immunoprecipitation were employed to detect and analyze the interactions between these two proteins.To assess cell cycle dynamics,we performed thymidine(TdR)double-blocking release assay and used nocodazole to block the cell cycle,with subsequent analysis via flow cytometry.Cell viability was measured by MTT assay.Western blot was conducted to evaluate the protein expression levels of ERα and ClC-3,while immunofluorescence staining was utilized to assess their subcellular distribution.RESULTS:(1)Anal-ysis from the web database revealed a significant correlation between ERα and ClC-3 expression,and co-immunoprecipita-tion confirmed their interaction.(2)We successfully obtained human breast cancer T47D cells at different cycle stages us-ing the TdR double-blocking release method and nocodazole treatment.(3)Treatment with TAM primarily inhibited T47D cell viability during G2/M phase.(4)Both ERα and ClC-3 exhibited cyclic variations in protein expression,with their sub-cellular distributions showing periodicity and co-localization.(5)Protein interactions between ERα and ClC-3 were ob-served across all cell cycle phases;(6)After TAM treatment,ERα expression peaked in G2/M phase,while ClC-3 expres-sion remained unaffected.CONCLUSION:Our findings demonstrate cyclic differences in the expression and distribution of ERα and ClC-3 in human breast cancer T47D cells,along with confirmed interactions between these two proteins.The cyclic properties of ERα may play a role in mediating the cell cycle specificity of TAM's anti-breast cancer effect.
