Nobiletin,an active ingredient of Fructus Aurantii,attenuates Ang Ⅱ-in-duced cardiac hypertrophy by inhibiting cardiomyocyte pyroptosis
10.3969/j.issn.1000-4718.2025.03.007
- VernacularTitle:枳壳活性成分川陈皮素通过抑制心肌细胞焦亡减轻Ang Ⅱ诱导的心脏肥大
- Author:
Min TAN
1
;
Jing CHEN
;
Yi ZENG
;
Jiayu CHEN
;
Xianqing WANG
;
Shan HUANG
;
Yi CAI
Author Information
1. 常德职业技术学院药学系,湖南 常德 415000
- Publication Type:Journal Article
- Keywords:
Fructus Aurantii;
nobiletin;
cardiac hypertrophy;
pyroptosis
- From:
Chinese Journal of Pathophysiology
2025;41(3):472-480
- CountryChina
- Language:Chinese
-
Abstract:
AIM:This study aimed to identify the key active components and signaling pathways in the tradi-tional Chinese medicine Fructus Aurantii that contribute to the prevention and treatment of cardiac hypertrophy,along with experimental validation.METHODS:H9C2 cardiomyocytes were pretreated with nobiletin(NOB)for 1 h and then ex-posed to 100 nmol/L angiotensin Ⅱ(Ang Ⅱ)for 24 h.RT-qPCR was used to quantify the mRNA expression of hypertrophy-related genes,including atrial natriuretic peptide(ANP),brain natriuretic peptide(BNP)and myosin heavy chain 7(MYH7).Immunofluorescence staining was employed to assess the surface area of cardiomyocytes.Additionally,a kit was utilized to measure levels of pyroptosis-related factors such as lactate dehydrogenase(LDH),interleukin-1β(IL-1β),IL-18 and caspase-1,while Western blot was performed to evaluate the expression of gasdermin D and caspase-1.RESULTS:Network pharmacology analyses indicated that NOB is the key active component in Fructus Aurantii that regu-lates cardiac hypertrophy,potentially through the pyroptosis pathway.Further molecular biology experiments confirmed that NOB inhibits Ang Ⅱ-induced cardiac hypertrophy and pyroptosis.Furthermore,the involvement of the pyroptosis pathway was highlighted in the protective effects of NOB against cardiac hypertrophy.CONCLUSION:The active compo-nent NOB in the traditional Chinese medicine Fructus Aurantii alleviates cardiac hypertrophy by inhibiting pyroptosis.
