miR-23c-targeted Octamer-binding Transcription Factor 1 Regulates Hepatocellular Carcinoma Cell Proliferation,Migration and Apoptosis
10.3870/j.issn.1672-0741.25.01.009
- VernacularTitle:miR-23c靶向八聚体结合转录因子1调控肝癌细胞增殖、迁移与凋亡
- Author:
Panpan XIA
1
;
Wei ZHANG
1
;
Sisi LI
1
Author Information
1. 武汉大学中南医院消化内科/湖北省肠病临床研究中心/肠病湖北省重点实验室,武汉 430071
- Publication Type:Journal Article
- Keywords:
hepatocellular carcinoma;
miR-23c;
octamer-binding transcription factor 1;
proliferation;
migration;
apoptosis
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2025;54(3):363-369
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of miR-23c and its target gene OCT1 on the proliferation,migration and apoptosis of hepatocellular liver cancer.Methods The expression level of miR-23c in normal liver cell line LO2 and hepatocel-lular carcinoma cell lines(including Huh7,HepG2,Hep-3B,SK-HEP1,HCCLM3 and MHCC97H)was detected by real-time flu-orescence quantitative PCR(qRT-PCR).The miR-23c mimics were designed to measure the proliferation status,migration abili-ty and apoptosis level of cells in the hepatocellular carcinoma cell line Huh7 using CCK-8 assay,cell migration assay and flow cytometric analysis.The target relationship between miR-23c and OCT1 was verified by predictive site prediction and dual lucif-erase assay,and the effect of miR-23c overexpression on OCT1 level was analyzed by Western blotting.The effect of miR-23c targeting OCT1 on the proliferation,migration and apoptosis of hepatocellular carcinoma was investigated by rescue as-say.Results Compared with the normal liver cell line LO2,the expression of miR-23c was significantly downregulated in liver cancer cell lines.In comparison with the NC group,Huh7 cells transfected with miR-23c mimics showed reduced proliferation and migration,while apoptosis was significantly enhanced.The interaction between miR-23c and OCT1 was verified.OCT1 pro-tein expression in Huh7 cells was reduced after miR-23c overexpression.The proliferation and migration of Huh7 cells were in-hibited,and apoptosis was promoted after transfection of miR-23c mimics.These effects were reversed by overexpression of OCT1.Conclusion miR-23c can downregulate OCT1,thereby inhibiting proliferation and migration,and promoting apoptosis in hepatocellular liver cancer cells.
