Characteristics of Intestinal Flora in Patients with Large Intestine Polyps were Analyzed by 16SrDNA Sequencing
10.11969/j.issn.1673-548X.2025.05.018
- VernacularTitle:基于16SrDNA测序法分析大肠息肉患者肠道菌群的特征
- Author:
Xinnian FU
1
;
Chi MA
;
Xinxin WANG
Author Information
1. 671003 大理大学
- Publication Type:Journal Article
- Keywords:
large intestine polyp;
Intestinal flora;
16SrDNA sequencing;
Advanced adenomatous polyps;
UBA1819
- From:
Journal of Medical Research
2025;54(5):93-98,165
- CountryChina
- Language:Chinese
-
Abstract:
Objective The changes and differences of intestinal flora in patients with non-adenomatous polyps,common adenoma-tous polyps and advanced adenomatous polyps were analyzed.Methods Stool samples of patients with large intestine polyps meeting the ataxic standard and healthy physical examination subjects were collected from September 2022 to December 2023 in the Department of Gastroenterology of the Second Affiliated Hospital of Dali University,and the intestinal flora sequencing data were analyzed by 16SrDNA gene sequencing method.Results The results of Alpha diversity index showed that the diversity and abundance of bacteria in non-ade-noma group,common adenoma group and advanced adenoma group were decreased compared with healthy control group(P＜0.05).At the phylum level,compared with the healthy group,the relative abundance of Proteobacteria increased in the non-adenoma group,the com-mon adenoma group and the advanced adenoma group,while the relative abundance of Bacteroidetes decreased.The relative abundance of campylobacter in advanced adenoma group was significantly lower than that in non-adenoma group and common adenoma group(P=0.005).At the genus level,the relative abundances of Escherichia coli Shigella and Streptococcus in the non-adenoma group,common adenoma group and advanced adenoma group were higher than those in the healthy control group,while the relative abundances of Fecalis praecalis and Agathobacter were lower than those in the healthy group(P＜0.05).The relative abundance of UBA1819 bacteria in the ad-vanced adenoma group was higher than that in the non-adenoma group and the common adenoma group(P=0.038).Conclusion In-testinal flora diversity and abundance decreased in patients with non-adenomatous polyps,common adenomatous polyps and advanced ad-enomatous polyps,while UBA1819 bacteria level increased in patients with advanced adenomatous polyps.
