PCSK9 promotes proliferation and invasion of ovarian cancer cells in vi-tro through MAPK/ERK pathway
10.3969/j.issn.1000-4718.2025.03.004
- VernacularTitle:PCSK9通过MAPK/ERK通路促进卵巢癌细胞体外增殖和侵袭的实验研究
- Author:
Minmin WU
1
;
Jie LUO
1
;
Fenger LIAO
1
;
Ting ZHENG
1
;
Di FAN
1
;
Qin GUO
1
Author Information
1. 广东药科大学附属第一医院妇产科,广东 广州 510062
- Publication Type:Journal Article
- Keywords:
ovarian cancer;
PCSK9 protein;
biomarkers;
MAPK/ERK signaling pathway
- From:
Chinese Journal of Pathophysiology
2025;41(3):444-452
- CountryChina
- Language:Chinese
-
Abstract:
AIM:This study aims to investigate the role and mechanism of proprotein convertase subtilisin/kexin type 9(PCSK9)in ovarian cancer.METHODS:We compared the expression levels of PCSK9 between ovarian cancer specimens and their corresponding adjacent non-cancerous tissues,while also assessing its expression in various ovarian cancer cell lines.Using a shRNA strategy,we reduced the expression of PCSK9 in ovarian cancer cell lines cul-tured in vitro,with confirmation via Western blot.The effects of PCSK9 downregulation on the proliferation,migration,and invasion of ovarian cancer cells were evaluated through EdU,colony formation,and Transwell assays.Additionally,we analyzed the impact of PCSK9 down-regulation on the MAPK/ERK signaling pathway using Western blot analysis.RE-SULTS:PCSK9 was significantly upregulated in ovarian cancer tissues and cell lines(P＜0.01).Downregulation of PC-SK9 resulted in a significant decrease in cell proliferation,migration,and invasion(P＜0.01).Western blot analysis dem-onstrated that PCSK9 knockdown led to reduced expression levels of key molecules within the MAPK/ERK signaling path-way(P＜0.01).CONCLUSION:PCSK9 promotes the proliferation and invasion of ovarian cancer cells by activating MAPK/ERK signaling pathway.
