Construction and Analysis of a Prognostic Risk Model for Lung Adenocarcinoma Based on m6A-related Genes
10.11969/j.issn.1673-548X.2025.05.024
- VernacularTitle:基于m6A RNA修饰相关基因的肺腺癌预后模型构建与分析
- Author:
Meng FANG
1
;
Fan YANG
1
;
Nongyan WANG
1
Author Information
1. 310013 杭州,中国人民解放军联勤保障部队第903医院(杭州医学院附属西湖医院)全科医学科
- Publication Type:Journal Article
- Keywords:
Lung adenocarcinoma;
m6A;
Prognosis;
Immune infiltration;
Driver-gene mutation
- From:
Journal of Medical Research
2025;54(5):129-135
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a prognostic model for lung adenocarcinoma(LUAD)based on N6-methyladenosine(m6A)-related genes and explore its clinical significance.Methods The LUAD dataset was obtained from the Cancer Genome Atlas(TCGA)and the Gene Expression Omnibus(GEO).The TCGA dataset was divided into a training set and a validation set in a 7∶3 ratio.In the training set,the m6A-related gene prognostic model was constructed using univariate COX analysis and LASSO regression analysis.Risk scores were calculated for the TCGA,GSE30219,GSE31210,GSE41271,GSE50081,and GSE68465datasets.Patients were categorized into low-risk and high-risk groups based on the median value of the risk scores.Kaplan-Meier(KM)curves,receiver operating char-acteristic(ROC)curves and multivariate COX regression analysis were used to verify the reliability of the prognosis model.A nomogram model was constructed to assess the role of risk scores in prognostic monitoring.Gene set enrichment analysis(GSEA)was performed to identify enriched signaling pathways in the prognostic model.The correlation between risk scores,immune infiltration,and driver gene mutations was analyzed.Results A prognostic model comprising nine m6A-related genes(AKAP12,CBFA2T3,KIF14,KL,KRT6A,LIFR,MIF,RRM2,TLR8)was developed.which emerged as an independent prognostic factor for LUAD.The model based on the risk score,T stages and N stages,accurately predicted LUAD prognosis.In high-risk group,mTORC1,Myc signaling pathways,and DNA repair mechanisms were significantly activated,the infiltration of CD8+T cells and CD4+T cells decreased,the expression of CD276 in-creased,and the expression of CTLA4 decreased.Additionally,risk scores were correlated with EGFR and KRAS mutations.Conclusion The prognostic model based on m6A-related genes effectively predicts LUAD prognosis and can guide targeted therapy and immunothera-py treatments for patients.
