The Mechanism of Xiaoding Ointment Regulating the NLRP3/NF-κB/Caspase-1 Pathway in Treatment of Acute Closed Soft Tissue Injury
10.3870/j.issn.1672-0741.24.12.034
- VernacularTitle:消定膏干预NLRP3/NF-κB/Caspase-1通路治疗急性闭合性软组织损伤的作用机制
- Author:
Yujuan WANG
1
;
Yonglong JIA
;
Jing LIU
Author Information
1. 甘肃中医药大学附属医院创伤骨科,兰州 730020
- Publication Type:Journal Article
- Keywords:
Xiaoding Ointment;
acute closed soft tissue injury;
pyroptosis;
NLRP3 inflammasome;
NF-κB signa-ling pathway;
Caspase-1;
inflammatory factor
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2025;54(3):328-334
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the molecular mechanism of Xiaoding Ointment in inhibiting pyroptosis through nucle-otide binding oligomerization of domain like receptor protein 3(NLRP3)/nuclear factor kappa B(NF-κB)/caspase-1 pathway,and to analyze its therapeutic effect on acute closed soft tissue injury.Methods Sixty SPF SD rats were selected and randomly divided into four groups:blank group,model group,positive control group(Qizheng Xiaotong plaster),and Xiaoding Ointment group(topical application).The blank group did not receive any treatment,while the other groups received corresponding inter-vention treatment after preparing the acute closed soft tissue injury model,with a treatment period of seven days.The conditions such as swelling,pain and limited movement were evaluated through the injury symptom score.Hematoxylin-eosin(HE)staining was used to observe the histopathological changes of rat leg muscle tissue.ELISA was used to detect the levels of serum inflam-matory factors IL-1β and TNF-α.TUNEL staining was used to observe the pyroptosis of cells.The expression levels of NLRP3,NF-κB,p65 and Caspase-1 proteins were analyzed by Western blotting and immunohistochemistry to evaluate the therapeutic effect and mechanism of action of Xiaoding Ointment.Results Compared with the model group,the swelling degree,appearance changes,activity and pain behavior of rats in the Xiaoding Ointment group were significantly improved(all P＜0.05).Histopa-thology showed a decrease in inflammatory cell infiltration and a normalization of muscle fiber arrangement.The levels of serum inflammatory factors IL-1β and TNF-α were significantly reduced(both P＜0.05);The number of pyroptosis positive cells and pyroptosis positive rate significantly decreased(both P＜0.05);The protein expressions of NLRP3,NF-κB,p65,and Caspase-1 was significantly reduced(all P＜0.05).Conclusion Xiaoding Ointment inhibits NLRP3 inflammasome activation and NF-κB signaling pathway through targeted inhibition,blocks Caspase-1-dependent apoptosis process,and reduces the release of inflam-matory factors such as IL-1β and TNF-α,thereby alleviating the inflammatory response after acute soft tissue injury and promo-ting tissue repair.
