Effects of sufentanil on proliferation invasion migration and apoptosis of thyroid cancer cells by regulating the AMPK-SIRT1-PGC-1α signaling pathway
10.3969/j.issn.1005-6483.20240399
- VernacularTitle:舒芬太尼调节AMPK-SIRT1-PGC-1α信号通路对甲状腺癌细胞增殖、侵袭迁移和凋亡的影响
- Author:
Tao MA
1
;
Xinxin LI
1
;
Juan QI
1
;
Airong ZHANG
1
Author Information
1. 061000 河北沧州,沧州市人民医院麻醉科
- Publication Type:Journal Article
- Keywords:
sufentanil;
AMPK-SIRT1-PGC-1α signaling pathway;
thyroid cancer;
proliferation;
migration;
invasion;
apoptosis
- From:
Journal of Clinical Surgery
2025;33(6):596-601
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of sufentanil on proliferation,invasion,migration,and apoptosis of thyroid cancer cells,and analyze whether its mechanism is related to the AMP-activated protein kinase(AMPK)-silent information regulator 1(SIRT1)-peroxisome proliferat or activator receptor gamma coactivator 1α(PGC-1α)signaling pathway.Methods The human thyroid cancer cell line(TPC-1)was separated into a control group,L,M,H-sufentanil groups,and H-sufentanil+AICAR group.TPC-1 cell activity,apoptosis,invasion,migration,and expression of proteins were detected using CCK-8 assay,flow cytometry,Transwell assay,scratch assay,and Western blot,respectively.Results Compared with the control group,the absorbance value,invasion number,scratch healing rate,cleaved-Caspase-3,programmed cell death 1 ligand 1,B lymphoblastoma-2,p-AMPK/AMPK,SIRT1,and PGC-1αprotein levels in L,M,and H-sufentanil groups were reduced;the apoptosis rate and Bax protein level were increased(P＜0.05).AICAR treatment reversed the effect of H-sufentanil on the above indicators(P＜0.05).Conclusion Sufentanil may inhibit the AMPK-SIRT1-PGC-1α signaling pathway,thereby inhibiting the proliferation and invasion migration of thyroid cancer cells,and promoting cell apoptosis.
