The effect and mechanism of valsartan on energy metabolism of mice
10.3969/j.issn.1006-7795.2025.01.017
- VernacularTitle:缬沙坦对小鼠能量代谢的影响及机制初探
- Author:
Wenyi JI
1
;
Lini SONG
;
Xi CAO
Author Information
1. 首都医科大学附属北京同仁医院北京市糖尿病研究所，北京 100730
- Publication Type:Journal Article
- Keywords:
angiotensin Ⅱ receptor antagonist;
glycolipid metabolism;
brown adipose tissue;
thermogenesis
- From:
Journal of Capital Medical University
2025;46(1):106-114
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect and possible mechanism of angiotensin Ⅱ receptor blocker(ARB)on energy metabolism in mice.Methods ① Male 6-week-old C57BL/6N mice were fed with normal diet(ND)and high-fat diet(HFD)for 6 weeks,then detect their body weight and fasting blood glucose.② The diabetic mice induced by high-fat diet were randomly divided into control group(Con)and drug treatment group(ARB).They were administered with saline and valsartan at a dose of 3 mg/kg per day via gastric lavage for 6 weeks.After that,the body weight,body temperature,intraperitoneal glucose tolerance test,respiratory metabolism rate,hematoxylin-eosin staining of adipose tissue,and the expression of thermogenesis-related genes in brown adipose tissue were examined.③By using male 6-week-old db/db mice as the experimental subjects,they were also administered with saline and valsartan at a dose of 3 mg/kg per day via gastric lavage for 5 weeks.After that,the body weight,fasting blood glucose,and body temperature were measured.Results High-fat diet successfully induced the diabetic mouse model.Valsartan treatment significantly reduced the weight gain rate of HFD mice and the body weight of db/db mice,increased the body temperature of HFD mice in a 4℃cold environment,and improved the energy expenditure of HFD mice,but had no significant improvement on glucose metabolism in HFD mice.Compared with the control group,the fasting blood glucose level of db/db mice was significantly reduced after valsartan treatment,and the body temperature showed an upward trend after 2 h and 3 h of cold treatment at 4 ℃,but the difference was not statistically significant.In addition,for HFD mice,valsartan treatment significantly reduced the cell volume of brown adipocyte,subcutaneous adipocyte,and epididymal adipocyte.At the mRNA level,valsartan promoted the expression of UCP1 in brown adipose tissue,while the expression of Prdm16,PGC1α and AP2 expression did not change significantly.Conclusions Valsartan can improve energy metabolism in obese mice,and its mechanism may be related to the positive effects of ARB on the thermogenesis of brown adipose tissue.
