Mechanisms by which EPB41L4A-AS1 Influences Glial Cells-mediated Aβ Clearance
10.13241/j.cnki.pmb.2025.12.003
- VernacularTitle:EPB41L4A-AS1影响神经胶质细胞清除Aβ的作用机制
- Author:
Li-xin NIU
1
;
Xu-fei ZHANG
1
;
Tian-zi LI
1
;
Ming-hui LI
1
;
Rui-xue YIN
1
;
Zi-qiang WANG
1
Author Information
1. 山东第一医科大学生物医学科学学院 山东济南 250017
- Publication Type:Journal Article
- Keywords:
EPB41L4A-AS1;
Transcriptome sequencing;
Glial cell;
Aβ
- From:
Progress in Modern Biomedicine
2025;25(12):1942-1947
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the changes in the whole transcriptome gene expression profile affected by EPB41L4A-AS,and to reveal its potential mechanisms that influence the progression of AD.Methods:U251 cells with stable low expression of EPB41L4A-AS1 were constructed using shRNA technology.Transcriptome sequencing was performed to screen for transcripts regulated by EPB41L4A-AS1.KEGG pathway and GO analysis were used to explore the related signaling pathways and biological processes regulated by EPB41L4A-AS1.Immunofluorescence assay was used to investigate the effects of EPB41L4A-AS1 on the activity of glial cells with antibodies against GFAP.Results:Knocking down the expression of EPB41L4A-AS1 in U251 cells significantly influenced the levels of multiple transcripts,with 626 upregulated and 949 downregulated.Further analysis revealed that the downregulated transcripts are related to AD,activation and proliferation of glial cells,and formation of amyloid fibers,and close to multiple signaling pathways that are involved in the glial cells-mediated Aβ clearance.Cellular experiments have shown that EPB41L4A-AS1 regulated the synapses length and activity of glial cells.Conclusions:EPB41L4A-AS1 may influence the glial cells-mediated Aβ clearance through multiple signaling pathways.
