Expression of CD99 in Breast Cancer and Its Effects on Cell Proliferation and Migration
10.3870/j.issn.1672-0741.24.12.025
- VernacularTitle:CD99在乳腺癌组织中的表达及对乳腺癌细胞增殖和迁移的影响
- Author:
Xinzhen DAI
1
;
Hongping TANG
;
Li YUAN
Author Information
1. 南方医科大学附属中山市博爱医院病理科,中山 528403
- Publication Type:Journal Article
- Keywords:
CD99;
breast cancer;
MDA-MB-435S cells;
cell proliferation;
cell migration
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2025;54(3):352-356
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of CD99 in breast cancer and its effects on proliferation and migration of MDA-MB-435S cell.Methods Expression of CD99 was detected by immunohistochemical examination in tissues from 80 cases of breast cancer,40 cases of ductal carcinoma in situ,60 cases of benign breast hyperplasia,and 20 cases of normal breast.A cell line MDA-MB-435S-SiCD99 and the negative control cell line MDA-MB-435S-SiNC were constructed,which were transfected with SiCD99 and its negative control.Western blot was used to detect the protein levels of CD99 after CD99 silencing in MDA-MB-435S cell.The effects of CD99 on MDA-MB-435S cell proliferation and migration were evaluated using cell counting kit-8(CCK-8),transwell and wound healing assays via silencing CD99 in MDA-MB-435S cell.Results The expression of CD99 in breast cancer tissue was higher than that in tissue of ductal carcinoma in situ,benign breast hyperplasia tissue and normal breast tissue(P＜0.01).Western blot result showed that the expression of CD99 was decreased after CD99 silencing in MDA-MB-435S cell(P＜0.01).Compared with the MDA-MB-435S-SiNC,the proliferation rate was lower(P＜0.01,CCK-8 test),the scratch distance was shorter(P＜0.01,wound healing assay),and the migration abilities was obviously decreased(P＜0.01,tr-answell assay)in MDA-MB-435S-SiCD99.Conclusion CD99 is upregulated in the breast cancer and silencing CD99 obviously inhibited the proliferation and migration of breast cancer cells,providing evidence for diagnosis and potential treatment of breast cancer.
