Construction of miR-181a-5p stable transfection OSCC cell strain and investigation of its effects on the in-vasion and migration of OSCC cells
10.3969/j.issn.1001-3733.2025.02.009
- VernacularTitle:miR-181a-5p过表达慢病毒载体构建及其对OSCC细胞侵袭和迁移的影响
- Author:
Rui XUE
1
;
Guoqiang XU
;
Junting YANG
;
Yiyan YANG
;
Xuehai WU
;
Guohua SONG
Author Information
1. 030000 太原,山西医科大学实验动物中心,实验动物与人类疾病动物模型山西省重点实验室
- Publication Type:Journal Article
- Keywords:
miR-181a-5p;
Stable cell line;
Oral cancer;
Migration;
Invasion
- From:
Journal of Practical Stomatology
2025;41(2):193-199
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct a stable oral squamous cell carinoma(OSCC)cell line overexpressing miR-181a-5p,and to ob-serve the effects of miR-181a-5p on the biological behavior of the cells.Methods:miR-181a-5p lentivirus and the control virus vec-tor were respectively constructed and respectively transfected into OSCC CAL-27 cells.The stablely transfected cells(OE)and con-trol cells(NC)were screened out by purinomycin.The fluorescence of the cells was observed by laser confocal microscopy.The ex-pression of miR-181a-5p and Bcl-2 was detected by qRT-PCR and the expression of target protein Bcl-2 was detected by Western blot.The invasion,migration and proliferation ability of the cells were detected by cell scratch,Transwell assay and clonal formation assay.qRT-PCR was used to detect the expression of the related genes of cell invasion and migration.Results:It was found that the expression level of miR-181a-5p in OE cells was significantly higher than that in NC and untransfected control cells(P＜0.01).The expression of Bcl-2 in OE cells was significantly decreased.The invasion,migration and proliferation of OE cells were decreased.In ad-dition,the expression levels of MMP2,MMP9,Vimentin and Ki67 in OE cells were decreased,while E-cadherin was increased(P＜0.01).Conclusion:Overexpression of miR-181a-5p can significantly inhibit the proliferation,invasion and migration OSCC cells.
