Application of multi-omics technologies for microbiota research in childhood caries
10.3969/j.issn.1001-3733.2025.02.023
- VernacularTitle:菌群微生物的多组学技术在儿童龋病研究中的应用
- Author:
Ming HOU
1
;
Xinwei LI
1
;
Shukai SUN
1
;
Lian WU
1
Author Information
1. 710032 西安,口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔疾病临床医学研究中心,空军军医大学口腔医院儿童口腔科
- Publication Type:Journal Article
- Keywords:
Early childhood caries;
Multi-omics technology;
Oral microbial plaque ecosystem;
Caries prevention
- From:
Journal of Practical Stomatology
2025;41(2):283-288
- CountryChina
- Language:Chinese
-
Abstract:
Dental caries is the most common oral disease in children,not only impairing chewing,speech,and other functions while also impacting maxillofacial development and overall health.Recent researches have established the"ecological plaque theory",which posits that an imbalance in the oral plaque microecology leads to the demineralization of teeth hard tissues,as the widely ac-cepted etiology of dental caries.However,the significant variability in the composition and function of children's plaque microorgan-isms,influenced by primary tooth eruption and replacement,changes in diet,and increase in social interactions,makes traditional culture methods inadequate for studying this complex micro-ecosystem.Advances in multi-omics technologies have recently created new opportunities for studiing complex microecology.High-throughput sequencing technologies,such as 16S rRNA gene sequencing and metagenomics,allow for the exploration of microbial diversity and the analysis of genomic functions and their associations with dental caries.Mass spectrometry-based technologies,including metabolomics and proteomics,might elucidate the molecular mecha-nisms of dental caries by analyzing differential metabolites and pathways.This review focuses on the application of multi-omics tech-nologies to explore the relationships between the structure,function,and metabolic state of oral microbial communities and pediatric dental caries.It further discusses the future of oral microorganisms as diagnosis and therapeutic targets for caries,and is expected to provide novel strategies for the prevention and treatment of pediatric dental caries.
