Phlorizin improves cecal inflammation and intestinal barrier damage induced by Salmonella typhimurium in mice
10.16303/j.cnki.1005-4545.2025.03.25
- VernacularTitle:根皮苷改善鼠伤寒沙门菌诱导的小鼠盲肠炎症以及肠道屏障损伤
- Author:
Tong LI
1
;
Lijuan KONG
;
Yanli FENG
;
Ji CHENG
;
Tianyuan JU
;
Jiawen HAN
;
Shoupeng FU
;
Juxiong LIU
;
Guiqiu HU
;
Haolong ZHANG
Author Information
1. 吉林大学动物医学学院,吉林长春 130062
- Publication Type:Journal Article
- Keywords:
phlorizin;
Salmonella typhimurium;
inflammatory factors;
intestinal barrier
- From:
Chinese Journal of Veterinary Science
2025;45(3):587-593
- CountryChina
- Language:Chinese
-
Abstract:
Forty adult male C57BL/6 mice were randomly divided into four groups:the control group,Salmonella typhimurium(S.typhimurium)(ST)group,phlorizin(PHZ)+S.typhimuri-um(ST)group,and PHZ(80 mg/kg)group,with 10 mice in each group.Morphological observa-tion,HE staining,ELISA,immunofluorescence and Western blot were performed,the results showed that PHZ significantly increased the cecal index,decreased the spleen index of S.typhi-murium-induced mice(P＜0.05),and reduce the pathological damage of cecum in mice.Mean-while,PHZ treatment also significantly reduced colonization of S.typhimurium in the cecum,spleen,mesenteric lymph nodes and liver(P＜0.05).The results of ELISA showed that PHZ treatment also significantly inhibited the S.ty phimurium-induced increase in the expression of IL-1β,INF-γ,TNF-a and IL-6 in the cecum of mice(P＜0.05).Immunofluorescence and Western blot results showed that PHZ significantly increased the protein expression levels of Occludin,Claudin-3,and ZO-1 in the cecal barrier of mice induced by S.typhimurium(P＜0.05).These results con-firmed that phlorizin could improve cecal inflammation and intestinal barrier damage induced by S.typhimurium in mice.
