Molecular epidemiological investigation of main intestinalprotozoa infection in BALB/c mice
10.16303/j.cnki.1005-4545.2025.03.16
- VernacularTitle:BALB/c小鼠肠道主要原虫感染情况的分子流行病学调查
- Author:
Yu ZHENG
1
;
Xichen ZHANG
1
;
Yao RONG
1
;
Jianhua LI
1
;
Pengtao GONG
1
;
Xiaocen WANG
1
;
Taotao YUE
1
;
Xin LI
1
;
Xu ZHANG
1
;
Nan ZHANG
1
Author Information
1. 吉林大学动物医学学院人兽共患病研究教育部重点实验室/人畜共患传染病重症诊治全国重点实验室,吉林长春 130062
- Publication Type:Journal Article
- Keywords:
Tritrichomonas muris;
Pentatrichomonas hominis;
Cryptosporidium tyzzeri;
BALB/c mice
- From:
Chinese Journal of Veterinary Science
2025;45(3):514-518
- CountryChina
- Language:Chinese
-
Abstract:
To understand the infection status of main intestinal protozoa in BALB/c mice and pro-vide a basis for further control of intestinal protozoa infection.Five hundred and forty BALB/c mice provided by four domestic suppliers of BALB/c mice were detected for intestinal protozoa,in which 140 from supplier A,130 from supplier B,135 from supplier C,and 135 from supplier D,re-spectively.Fresh faecal samples were collected from each mouse separately to extract the genome and amplified by nested PCR based on primers for the 18S rRNA gene sequences of Pent-atrichomonas hominis(P.hominis)and Cryptosporidium tyzzeri(C.tyzzeri),and the 16S-like rRNA gene sequence of Tritrichomonas muris(T.muris)and sequenced.The results showed that the total intestinal protozoan infection rate was 7.1％(10/140)in 140 mice faecal samples provided by supplier A.Among them,the positivity rate of T.muris was 7.1％(10/140),C.tyzzeri was 2.1％(3/140),and P.hominis was 7.1％(10/140),the co-infection rate of two intestinal protozoa was 7.1％(10 mice:T.muris+P.hominis),and three intestinal protozoa was 2.1％(3 mice:T.muris+P.hominis+C.tyzzeri).The total intestinal protozoan infection rate in 135 mice faecal samples provided by supplier C was 7.4％,in which,7.4％(10/135)was positive for T.muris.There are no intestinal protozoa to be detected in 130 mice faecal samples from supplier B and 135 mice faecal samples from supplier D.The homology analysis showed that the homology of ampli-fied sequence of T.muris,P.hominis and C.tyzzeri was 98.52％,98.27％and 99.87％compared with published sequence of GenBank No:AY886846.1,GenBank No:AF156964.1 and GenBank No:KJ000486.1,which was clustered as an independent branch by phylogenetic analysis respec-tively.In conclusion,there are intestinal protozoan infection in BALB/c mice in some animal sup-pliers.The co-infections of more than 3 parasites such as T.muris,P.hominis and C.tyzzeri has been found.It will provide a basis for control of intestinal protozoa infection in BALB/c mice in the future.
