Analysis of infiltration of neutrophils and macrophages in tissues of eosinophilic gastroenteritis mice
10.3969/j.issn.1006-7795.2025.02.017
- VernacularTitle:中性粒细胞和巨噬细胞在嗜酸性胃肠炎小鼠各组织中的浸润情况分析
- Author:
Longwei XU
1
;
Yadan WANG
;
Minsi ZHOU
;
Jing WU
Author Information
1. 首都医科大学附属北京世纪坛医院消化内科,北京 100038
- Publication Type:Journal Article
- Keywords:
mice;
eosinophilic gastroenteritis;
eosinophils;
neutrophils;
macrophages
- From:
Journal of Capital Medical University
2025;46(2):306-313
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the infiltration of neutrophils and macrophages in the tissues of eosinophilic gastroenteritis mice.Methods Thirty female C57BL/6 mice aged 6-8 weeks were selected to induce the model of eosinophilic gastroenteritis in wild-type mice.They were sensitized by intraperitoneal injection of ovalbumin(OVA)protein and they were gastric stimulated with OVA.The successful modeling of eosinophilic gastroenteritis was verified by measuring the weight change of mice,detecting OVA specific immunoglobulin E(IgE)and Immunoglobulin G(IgG)levels in peripheral blood of mice by enzyme-linked immunosorbent assay(ELISA),and detecting intestinal eosinophils count by flow cytometry.The proportion of neutrophils and monocytes in peripheral blood and the proportion of neutrophils and macrophages in spleen,mesenteric lymph nodes,small intestine and colon tissues were detected by flow cytometry.Results The mice model of eosinophilic gastroenteritis was successfully induced by OVA abdominal sensitization and gavage stimulation.In mice with eosinophilic gastroenteritis,the infiltration of monocytes in peripheral blood,macrophages infiltration of spleen,mesenteric lymph nodes,small intestine and colon tissues were increased.And the neutrophil were increased infiltrated in peripheral blood,small intestine and colon tissues.Conclusion Macrophage and neutrophil were increased infiltrated in peripheral blood,intestinal tissue of mice with eosinophilic gastroenteritis.Furthermore,the infiltration of macrophage was increased in lymphoid tissues,such as spleen and mesenteric lymph nodes of mice with eosinophilic gastroenteritis.
