Regulation of white adipose tissue in mice by immunization with recombinant Bacillus Calmette-Gue?rin with c-di-AMP adjuvant
10.3969/j.issn.1002-2694.2025.00.002
- VernacularTitle:c-di-AMP为佐剂的重组卡介苗免疫对小鼠白色脂肪的调节作用
- Author:
Meng-juan DONG
1
;
Yu-xiao CHANG
;
Huan-huan NING
;
Yan-zhi LU
;
Jian KANG
;
Ming-ze XU
;
Ting DAI
;
Jia-ling LI
;
Le-ran HAO
;
Lin-na ZHANG
;
Yin-lan BAI
Author Information
1. 宁夏医科大学基础医学院生理学和神经生物学系,银川 750001;空军军医大学基础医学院微生物与病原生物学教研室,西安 710032
- Publication Type:Journal Article
- Keywords:
Bacillus Calmette-Gue?rin(BCG);
recombinant BCG(rBCG);
cyclic di-adenosine monophosphate(c-di-AMP);
white adipose;
lipid metabolism;
cytokines
- From:
Chinese Journal of Zoonoses
2025;41(4):370-375
- CountryChina
- Language:Chinese
-
Abstract:
This study assessed the role and mechanism of the recombinant Bacillus Calmette-Gue?rin vaccine(rBCG)with c-di-AMP adjuvant in regulating metabolism and immunity in epididymal white adipose(eWAT)in mice.Male C57BL/6 mice were intravenously immunized with BCG and rBCG,and their body weights were monitored.eWAT was isolated from the mice,and the stromal vascular fractions(SVFs)cell number was counted with a hemocytometer.Sections of mouse adipose tissue were prepared,and the size,number,and morphology of eWAT adipocytes and crown-like structure(CLS)formation were compared under a microscope after HE staining.The transcription levels of lipid metabolism-associated factors,cytokines and aging-associated genes in each group were determined with qRT-PCR.The body weights of mice gradually increased after immunization with BCG and rBCG.The proportions of eWAT increased,and the SVFs cell number decreased,in rBCG immunized mice.HE staining indicated that BCG immunization promoted hyperplasia,whereas rBCG immunization promoted hypertrophy of eWAT adipocytes;moreover,both BCG and rBCG immunization induced CLS formation in eWAT.The qRT-PCR results indicated that rBCG immunization inhibited the expression of genes associated with lipolysis and energy expenditure in eWAT.BCG immunization had little effect on cytokine transcription,whereas rBCG significantly induced the transcription of IFN-γ and IL-1Ra,and inhibited that of IL-15 and IL-2,but did not induce the expression of aging-associated genes.Thus,rBCG immunization induced eWAT adipocyte hypertrophy,which was associated with the inhibition of eWAT lipolysis and the regulation of cytokine expression.
