Effects of Pulsatilla powder on intestinal flora and tryptophan metabolism in co-lon of piglets with diarrhea
10.16303/j.cnki.1005-4545.2025.03.23
- VernacularTitle:白头翁散对泄泻仔猪结肠肠道菌群及肠道色氨酸代谢的影响
- Author:
Shuo FENG
1
;
Lifang ZHANG
1
;
Yufei XIE
1
;
Shengming JIANG
1
;
Liting CAO
1
;
Shicheng BI
1
;
Qi MA
1
Author Information
1. 西南大学动物医学院,重庆荣昌 402460
- Publication Type:Journal Article
- Keywords:
diarrhea;
Pulsatilla powder;
colonic tissue;
intestinal flora;
tryptophan metabolism
- From:
Chinese Journal of Veterinary Science
2025;45(3):568-579
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to investigate the effects of Pulsatilla powder(PP)on colonic intestinal flora and tryptophan metabolism in piglets with diarrhea.Twenty-four weaned piglets were ran-domly divided into the normal control group,model group,self-healing group,and PP group.The model of piglet diarrhea was established by the compound factor of external evil+internal injury+epi-demic virus,and treated with PP.During the modeling period,the body weight,mental status and fecal patterns of piglets were monitored daily.After the experiment,the colon tissues were collect-ed for histopathological observation,and the inflammatory factors IL-1β and IL-6 in the colon tis-sue was detected by ELISA,16S rRNA was used to analyze colonic intestinal flora and RT-qPCR was used to determine the expression of genes related to tryptophan metabolism in the colon.The results showed that,compared with the model group,PP significantly improved the pathological status of the colon in piglets with diarrhea,with elevated body mass and a significant increase in goblet cells(P＜0.05);and significantly reduced the levels of IL-1β and IL-6 in the colon of diar-rhea piglets(P＜0.05).The results of intestinal flora showed that PP increased the abundance of Firmicutes(P＜0.01),Spirochaeota(P＜0.01)and Lactobacillus(P＜0.05),reduced the abun-dance of Bacteroidota(P＜0.05)、Prevotella_NK3B31_group(P＜0.05)and Clostridium_sensu_stricto 1(P＜0.01).Meanwhile,PP significantly reduced the expression level of TPH1 mRNA(P＜0.05),increased the expression levels of AhR(P＜0.05)and IL-22 mRNA in the co-lon of diarrhea piglets.These results indicates that PP may alleviate diarrhea in piglets by regula-ting colonic intestinal flora and genes related to intestinal tryptophan metabolism.This study pro-vides data support for the subsequent investigation of piglet diarrhea prevention and treatment.
