Pathogenetic analysis of a case of mixed Pasteurella multocida and non-tuberculous mycobacteria infection in the lungs
10.3969/j.issn.1002-2694.2025.00.051
- VernacularTitle:1例多杀巴斯德氏菌混合非结核分枝杆菌肺部感染病例的病原学分析
- Author:
Wen-ying ZHENG
1
;
Shuo YE
Author Information
1. 宁波市第四医院,宁波 315700
- Publication Type:Journal Article
- Keywords:
Pasteurella multocida;
zoonotic diseases;
next-generation sequencing;
evolutionary tree
- From:
Chinese Journal of Zoonoses
2025;41(4):441-446
- CountryChina
- Language:Chinese
-
Abstract:
This study reports a case of pulmonary infection by Pasteurella multocida together with nontuberculous mycobacteria,and the pathogenic characteristics of P.multocida isolated in this case.The patient was a 69 year old woman admitted with bronchiecta-sis and infection.Bacterial culture of bronchoalveolar lavage fluid,tuberculosis culture,and Dano-seq multiple pathogen detection were performed after admission.Drug sensitivity testing and second-generation sequencing were performed on the isolated P.multo-cida strain.Non-tuberculous mycobacteria(M.kansasii)and P.multocida were detected in the bacterial culture and Dano-seq re-sults.The major virulence factors of this P.multocida strain included htrB,rfaE,and msbA,among a total of 21 factors.The resistance genes of this strain included floR,sul2,and FosA3,among ten identified genes.MLST analysis classified the P.multocida strain as ST12.A phylogenetic tree indicated that the strain was homologous to the main epidemic strains in China.A detection scheme combin-ing traditional isolation and cultivation with next-generation sequencing enables rapid and accurate identification of rare pathogens,and provides the necessary antimicrobial susceptibility results,thereby facilitating the accurate diagnosis and treatment of rare dis-eases.
