Effects of Pulsatilla decoction on 5-HT signaling system in the hippocampus of rats with damp-heat diarrhea
10.16303/j.cnki.1005-4545.2025.03.22
- VernacularTitle:白头翁汤对湿热泄泻大鼠海马区5-HT信号系统的影响
- Author:
Yunqi QU
1
;
Shengming JIANG
1
;
Shuo FENG
1
;
Chenying WANG
1
;
Siyue LAI
1
;
Qi MA
1
Author Information
1. 西南大学动物医学院,重庆荣昌 402460
- Publication Type:Journal Article
- Keywords:
Pulsatilla decoction;
damp-heat diarrhoea;
hippocampus;
5-HT signaling pathway
- From:
Chinese Journal of Veterinary Science
2025;45(3):559-567
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to investigate the effects of Pulsatilla decoction(PD)on the 5-HT sig-naling system in the hippocampus of damp-heat diarrhoea(DHD)rats.Twenty-four male SD rats were randomly divided into four groups including the blank group,the model group,the PD group and the self-healing group.Except for the blank group,the rats in each group were induced by"high sugar and high fat,high temperature and high humidity,and E.coli poisoning"to establish a of rat model DHD,and were treated by gavage with PD.Changes in body weight,temperature,food intake and water intake,routine blood tests and histopathological changes in the colon were recor-ded to comprehensively determine the modelling condition of rats with DHD;histopathological changes in the hippocampus of rats were observed,and real-time fluorescence PCR was used to de-termine the expression of IL-1β3,IL-6,TNF-α,IFN-γ and TPH1 mRNA in the hippocampus;West-ern blot and immunohistochemistry were used to detect the protein expression of tryptophan hydroxylase 1(TPH1),receptors(5-HT3R,5-HT4R,5-HT7R)in 5-HT signaling pathway in the hippocampus.The results showed that:PD significantly regulated the abnormal changes of body weight,food and water intake and blood routine indexes in rats with DHD,and significantly im-proved the pathological damage of colonic tissues;PD significantly lowered the expression of in-flammatory cytokines IL-1β,IL-6,IFN-γ,and TNF-α in hippocampus of rats with DHD(P＜0.05),and significantly reduced the expression of TPH1 mRNA in hippocampus of rats with DHD(P＜0.05).PD could increased the expression of 5-HT4R and 5-HT7R in the hippocampus of rats with DHD;reduced the expression of 5-HT3R and TPH in the hippocampus,among which 5-HT3R expression was significantly reduced.This study suggests that PD can affect the function of hippocampus in rats with DHD by regulating the 5-HT signaling pathway.
