Interaction of autophagy and ubiquitin proteasome system after macrophage infec-ted Brucella
10.16303/j.cnki.1005-4545.2025.03.15
- VernacularTitle:自噬与蛋白酶体途径在布鲁菌感染巨噬细胞过程中的相互作用
- Author:
Bingmei DONG
1
;
Mengmeng LIANG
;
Wenya XU
;
Jun WANG
;
Lili SONG
;
Honglin REN
Author Information
1. 沧州师范学院生命科学学院,河北沧州 061001
- Publication Type:Journal Article
- Keywords:
autophagy;
ubiquitin-proteasome system;
Brucella;
macrophage
- From:
Chinese Journal of Veterinary Science
2025;45(3):507-513
- CountryChina
- Language:Chinese
-
Abstract:
In order to explore the relationship between ubiquitin proteasome system(UPS)and au-tophagy after macrophage was infected with Brucella.On the one hand,the levels of LC3 Ⅱ、P62 and 20S proteasomes in cell supernatant and peritoneal fluid were determined respectively after RAW264.7 cells were infected and BALB/c mice were intraperitoneal inoculated with Brucella suis(B.suis).The results displayed that UPS was activated firstly,followed by autophagy after B.suis infection.On the other hand,we prepared the UPS-inhibited-cells and ALP-inhibited-cells by lacta-cystin and 3-methy ladenine(3-MA),and then the cells were infected by B.suis and the levels of LC3 Ⅱ、P62 与 20S proteasomes in cell supernatant were determined.The results showed that the ALP function was significantly improved when the effect of M
