Construction of infectious clone of genotype Ⅰ Japanese encephalitis virus GX strain
10.16303/j.cnki.1005-4545.2025.03.12
- VernacularTitle:基因Ⅰ型日本脑炎病毒GX毒株感染性克隆的构建
- Author:
Mengxue YAN
1
;
Jing YE
1
;
Shengbo CAO
1
;
Junyao XIONG
1
Author Information
1. 华中农业大学农业微生物国家重点实验室,湖北武汉 430070
- Publication Type:Journal Article
- Keywords:
genotype Ⅰ;
Japanese encephalitis virus;
infectious clone;
replication;
pathogenicity
- From:
Chinese Journal of Veterinary Science
2025;45(3):482-488
- CountryChina
- Language:Chinese
-
Abstract:
The infectious clone plasmid of genotype Ⅰ Japanese encephalitis virus GX strain was suc-cessfully obtained by reverse transcription-polymerase chain reaction,where the cDNA of GX strain is divided into three fragments for amplification and the three fragments were sequentially cloned into pBR322 vector.After the infectious clone plasmid pGX was sequenced correctly,the pGX and pCAGGS-T7 eukaryotic plasmid were co-transfected into BHK-21 cells for virus rescue.The experimentalresults indicated that the Japanese encephalitis virus could be successfully res-cuedfrom BHK-21 cells.The plaque experiment and mouse experiment indicated that the rescued virus had similar replication ability and pathogenicity with wild type virus.It was confirmed that the infectious clone of genotype Ⅰ Japanese encephalitis virus GX strain was successfully construc-ted in this study.
