Biological characteristics and control effect of a Salmonella typhimurium bacteri-ophage
10.16303/j.cnki.1005-4545.2025.03.14
- VernacularTitle:1株鼠伤寒沙门菌噬菌体的生物学特性及其防制效果
- Author:
Qishan SONG
1
;
Zhijie SONG
;
Xiaoqian WANG
;
Min ZHAO
;
Lulu LI
;
Yuqing LIU
;
Yujun ZHAO
Author Information
1. 山西农业大学 动物医学学院,山西晋中 030801;山东省农业科学院畜牧兽医研究所中英噬菌体工程联合实验室,山东济南 250000
- Publication Type:Journal Article
- Keywords:
Salmonella typhimurium;
bacteriophages;
physiological haracteristics;
biological control
- From:
Chinese Journal of Veterinary Science
2025;45(3):498-506
- CountryChina
- Language:Chinese
-
Abstract:
A Salmonella bacteriophage,PJN025,was isolated from wastewater and characterized for its biological properties,including whole-genome sequencing and assessment of therapeutic poten-tial in animal infection models.Transmission electron microscopy classified it within the Caudovi-ricetes family.PJN025 selectively lysed S.typhimurium and S.enteritidis with an incubation period of approximately 10 min,a burst time of 80 min,and an average burst size of approximately 132 PFU/cell,demonstrating effective bactericidal activity.The phage showed robust stability at temperatures ranging from 30 to 70 ℃ and pH levels from 3 to 12.Whole-genome sequencing re-vealed a complete genome length of 46 478 bp with a G+C content of 45.9％,consisting of 82 open reading frames and one tRNA.No known virulence or resistance genes were found.In Galleria mellonella infection model,a survival rate of 70％was achieved with a preventative dose of 1 × 108 PFU/mL PJN025,compared to a 5％survival rate in the positive control group after 48 h of bacterial challenge.In SPF mice infected with S.typhimurium via intraperitoneal injection,all mices in the positive control group died within five days.However,in the co-infection group treated with 1×108 PFU/mL PJN025,a promising survival rate of 60％was observed.This study showed that the bacteriophage PJN025 had strong specificity,high lytic efficiency,good acid and alkali tol-erance,strong heat resistance,and good safety and prevention effects,providing materials and ex-perimental basis for the development of subsequent phage products.
