Passive stretching improves aging skeletal muscle cells by regulating the AMPK/PGC-1α/FOXO3 signal-ing pathways
10.3969/j.issn.1001-1242.2025.06.003
- VernacularTitle:被动牵张运动通过调控AMPK/PGC-1α/FOXO3信号通路对衰老骨骼肌细胞的影响
- Author:
Chaoyang GUO
1
;
Yijie LIU
;
Shuting LIU
Author Information
1. 上海中医药大学附属岳阳中西医结合医院,上海市,201619;同济大学附属养志康复医院(上海市阳光康复中心)
- Publication Type:Journal Article
- Keywords:
passive stretching;
sarcopenia;
mitophagy;
mitochondrial function;
adenylate-activated protein kinase
- From:
Chinese Journal of Rehabilitation Medicine
2025;40(6):814-821
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of passive distraction training modalities on aging skeletal muscle cells and its underlying mechanisms,and to provide experimental data on effective interventions for patients with severe loss of locomotor capacity in severe skeletal sarcopenia.Method:An in vitro skeletal muscle cell aging model by C2C12 differentiated skeletal muscle cells with D-ga-lactose stimulation were established.Aging skeletal muscle cells were subjected to cyclic stretch(0.5Hz,24h)using an FX-5000 Flex Cell Tension system.Senescence and oxidative stress levels were assessed through mito-chondrial membrane potential,antioxidant content,EdU assay,and β-galactosidase staining.The expression of AMPK/PGC-α/FOXO3 signaling molecules related to muscle cell protein synthesis and decomposition in the cells was analyzed by Western Blot.Result:Passive stretching significantly reduced the positive rate of aging in senescent skeletal muscle cells(18.03%±6.82%)compared with the aging group(42.86%±10.54%,P<0.05).The expression levels of intracellu-lar AMPK and PGC-1α significantly increased after passive stretching,while the expression of FOXO3 de-creased significantly compared to the aging group(P<0.05).In addition,passive stretching increased the mito-chondrial membrane potential but appeared to reduce the antioxidant content in aging skeletal muscle cells,po-tentially related to changes in PGC-1α expression levels.Conclusion:Passive stretching improved the aging state of skeletal muscle cells by modulating the AMPK/PGC-1α/FOXO3 signaling pathway and oxidative stress levels,revealing the great potential of passive stretch-ing as the treatment for severe sarcopenia.
