Electroacupuncture modulates ATPase to alleviate detrusor muscle contraction after suprasacral spinal cord injury
10.3969/j.issn.1001-1242.2025.06.002
- VernacularTitle:电针调节大鼠三磷酸腺苷酶缓解骶上脊髓损伤后膀胱逼尿肌收缩的研究
- Author:
Qirui QU
1
;
Lifen ZHAN
1
;
Liya TANG
1
Author Information
1. 湖南中医药大学针灸推拿与康复学院,湖南省 长沙市,410208
- Publication Type:Journal Article
- Keywords:
electroacupuncture;
neurogenic bladder;
adenosine triphosphatase;
adenosine triphosphatase;
detru-sor muscle
- From:
Chinese Journal of Rehabilitation Medicine
2025;40(6):806-813
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the impact of electroacupuncture(EA)on adenosine triphosphatase(ATPase),ade-nosine triphosphate(ATP),phosphorylated myosin light chain kinase(p-MLCK),and phosphorylated myosin light chain(p-MLC)in the detrusor muscle of neurogenic bladder patients who have experienced suprasacral spinal cord injury(SSCI).The primary objective of this research was to elucidate the mechanism by which EA inhibits detrusor hyperreflexia in these patients.Method:Forty female SD rats were randomly divided into two groups:a control group and a sham operation group,each comprising eight rats.The remaining twenty-four rats were used to create a neurogenic bladder model with incontinence.The rats were then randomly divided into two groups:a model group and an elec-troacupuncture group,with eight rats in each group.Subsequently,the remaining twenty-four rats were used to create an incontinence-type neurogenic bladder model using a modified T10 spinal cord transection method.The rats were further divided into a model group and an electroacupuncture group,with eight rats in each group.On the nineteenth postoperative day,electroacupuncture was administered at the"Ciliao","Zhongji",and"Sanyinjiao"points for seven consecutive days.Urodynamic tests were conducted after the treatment,and the levels of ATPase,ATP,adenosine diphosphate(ADP),and adenosine monophosphate(AMP)in the detru-sor muscle were measured using enzyme-linked immunosorbent assay(ELISA).The levels of p-MLCK and p-MLC2 in the detrusor muscle were measured using Western Blot.Result:The model group exhibited higher abdominal leakage point pressure and maximum bladder pressure,as well as lower maximum bladder capacity,compared to the control group(P<0.01).Additionally,the ATP content in the detrusor muscle was higher,while the levels of ATPase,ADP,and AMP were lower(P<0.01).The p-MLC2 level in the detrusor muscle was higher,and the p-MLCK level was lower(P<0.01).In con-trast,the electroacupuncture group displayed lower maximum bladder pressure,reduced abdominal leakage point pressure,and increased maximum bladder capacity compared to the model group(P<0.01).The ATP con-tent in the detrusor muscle was lower,and the levels of ATPase,ADP,and AMP were higher(P<0.01).Fur-thermore,the p-MLC2 levels in the detrusor muscle were lower,and the p-MLCK level was higher(P<0.01).Conclusion:Electroacupuncture at"Ciliao","Zhongji",and"Sanyinjiao"acupoints effectively improves bladder function in rats with detrusor overactivity following SSCI.The underlying mechanism is associated with the up-regulation of ATPase expression in the detrusor muscle,promoting ATP hydrolysis and modulating p-MLCK and p-MLC2 levels,thereby inhibiting detrusor muscle contraction.
