Metabolomics of oropharyngeal specimens from postoperative pneumonia patients with gastric cancer
10.12138/j.issn.1671-9638.20256645
- VernacularTitle:胃癌术后肺炎患者口咽标本代谢组学研究
- Author:
Bizhen CHEN
1
;
Zimeng LI
1
;
Yaxin JIANG
1
;
Tingyu WEI
1
;
Rui JIA
1
;
Chao SHI
1
Author Information
1. 福建中医药大学附属第二人民医院医院感染管理处,福建 福州 350001
- Publication Type:Journal Article
- Keywords:
gastric cancer;
postoperative pneumonia;
oropharyngeal metabolites;
non-targeted metabolomics
- From:
Chinese Journal of Infection Control
2025;24(3):372-380
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the characteristics of oropharyngeal metabolites of postoperative pneumonia(POP)and non-POP patients with gastric cancer by non-targeted metabolomics,and to provide reference for revea-ling the pathogenesis as well as clinical prevention and control.Methods Oropharyngeal swabs were collected from patients who underwent surgical treatment for gastric cancer in the department of gastrointestinal surgery of a tertia-ry hospital in Fujian Province from January 2022 to December 2023,nested case-control study was adopted,with 30 cases in POP group and non-POP group,respectively,ultrahigh performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)technique was adopted for detection,and multivariate statistical analysis was per-formed.Results The metabolites of POP group and non-POP group preoperatively,POP group and non-POP group postoperatively,POP group preoperatively and postoperatively,as well as non-POP group preoperatively and post-operatively did not show a clear trend of separation on the score graph of principal component analysis(PCA),par-tial least squares discriminant analysis(PLS-DA),and orthogonal partial least squares discriminant analysis(OPLS-DA),but the screening of the differential metabolites revealed that the relative abundances of ascorbic acid and lipoic acid in the POP group preoperatively were lower than non-POP group preoperatively.The relative abundance of 5'-adenylic acid in the POP group preoperatively was lower than postoperatively,while the relative abundances of suc-cinic acid and phenylacetaldehyde were higher.There were differences in metabolic pathways such as phenylalanine.Conclusion Alteration of metabolite level due to abnormalities in specific metabolic pathways presents in the deve-lopment of gastric cancer POP and may be related to the development of POP.
