Arbutin alleviates the inhibitory effect of LPS on osteogenic differentiation of human periodontal ligament stem cells through the NF-κB signaling pathway
10.13591/j.cnki.kqyx.2025.05.005
- VernacularTitle:熊果苷通过NF-κB信号通路减轻脂多糖对人牙周膜干细胞成骨分化的抑制作用
- Author:
Jingping GAO
1
;
Linglu JIA
;
Hongning LIANG
;
Yong WEN
Author Information
1. 山东大学齐鲁医学院口腔医学院口腔医院种植科,山东省口腔组织再生重点实验室,口腔生物材料与组织再生山东省工程研究中心,山东省口腔疾病临床医学研究中心,山东济南(250012);德州市陵城区人民医院口腔科,山东德州(253500)
- Publication Type:Journal Article
- Keywords:
periodontal ligament stem cells;
arbutin;
periodontitis;
osteogenesis;
NF-κB
- From:
STOMATOLOGY
2025;45(5):347-354
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of arbutin(Arb)on osteogenic differentiation of human periodontal ligament stem cells(hPDLSCs)under inflammatory conditions and the mechanism of NF-κB signaling pathway in this process.Methods The effects of Arb on the proliferation and osteogenic differentiation of hPDLSCs were analyzed by CCK-8,alkaline phosphatase staining,Alizarin Red staining,and Western blot.After establishing an inflammatory model using lipopolysaccharide(LPS),the effect of Arb on the ex-pression levels of inflammatory factors in hPDLSCs was analyzed by RT-qPCR.The effect of Arb on osteogenic differentiation of hP-DLSCs was analyzed,and the effect of Arb on the NF-κB pathway was analyzed by Western blot.After adding the NF-κB signaling pathway activator PMA to the culture system,whether the effect of Arb on hPDLSCs changed was analyzed.Results 100 nmol/L Arb did not affect the proliferation of hPDLSCs,but significantly promoted cell osteogenic differentiation and inhibited the expression of in-flammatory factors under LPS stimulation.Arb reduced the activation effect of LPS on the NF-κB signaling pathway and the inhibitory effect on cell osteogenic differentiation,while the efficacy of Arb was partially eliminated by PMA.Conclusion Arb alleviates the in-hibitory effect of LPS on osteogenic differentiation of hPDLSCs by inhibiting NF-κB signaling.
