Study on the Distribution and Infiltration Characteristics of Tissue Resident Memory T Cells in Esophageal Squamous Cell Carcinoma and Its Relationship with Immunotherapy Prognosis
10.3969/j.issn.1671-7414.2025.02.003
- VernacularTitle:食管鳞状细胞癌组织中组织定居记忆T细胞分布浸润特征及与免疫治疗预后的关系研究
- Author:
Shouping ZHONG
1
;
Yanzheng HU
Author Information
1. 西安市中心医院心胸外科,西安 710003
- Publication Type:Journal Article
- Keywords:
esophageal squamous cell carcinoma;
tumor infiltrates lymphocytes;
tissue-resident memory;
immunotherapy
- From:
Journal of Modern Laboratory Medicine
2025;40(2):11-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution and infiltration characteristics of tissue-resident memory(TRM)cells in esophageal squamous cell carcinoma(ESCC),and further analyze relationship between TRM cell infiltration and ESCC immunotherapy and prognosis.Methods From January 2017 to December 2019,30 ESCC patients who did not receive preoperative neoadjuvant therapy in Xi'an Central Hospital were selected as focal tissue and para cancer tissue samples and peripheral venous blood samples.Immunofluorescence staining and flow cytometry were used to detect the distribution,expression infiltration of CD103+CD8+TRM cells and the expression of immune checkpoint molecules[Programmed death-1(PD-1)and T cell immunoglobulin and mucin domain 3(Tim-3)]in all samples.Another 86 ESCC patients who received neoadjuvant immune checkpoint PD-1 inhibitor treatment before surgery were selected during the same period,and the postoperative tissue samples were taken to detect the level of CD103+CD8+TRM cell infiltration,and the relationship between it and immunotherapy efficacy and prognosis was analyzed.Results The results of 30 ESCC patients who did not receive neoadjuvant therapy before surgery showed that:① CD103 was mainly localized in the cell membrane of tumor-infiltrating CD8+T lymphocytes.Most of the CD103+cells in ESCC cancer focus tissue co-express CD8+cells,while most of the CD103+cells in paracancer tissue did not co-express CD8+cells.②The proportion of CD103+CD8+TRM cells to CD8+T cells in ESCC cancer focus tissues was 76.9%±4.4%,which was significantly higher than that in adjacent normal tissues 65.8%±3.6%,and the difference was statistically significant(t=18.107,P＜0.001).③The proportion of CD103+CD8+TRM cells in ESCC tumor-infiltrating lymphocytes was 64.8%±4.3%,which was significantly higher than that in para-carcinoma infiltrating lymphocytes 34.6%±3.4%,the difference was statistically significant(t=30.175,P＜0.001),and peripheral blood lymphocytes were almost not expressed(1.1%±0.2%).④The immune checkpoint molecules PD-1 and Tim-3 were highly expressed in CD103+CD8+TRM cells of ESCC cancer foci.A sample of 86 ESCC patients treated with neoadjuvant immune checkpoint PD-1 inhibitors before surgery found that:① The proportion of CD103+CD8+TRM cell infiltration in the immunotherapy effective group was 76.5%±7.3%,which was significantly higher than that in immunotherapy ineffective group 58.7%±5.8%,the difference was statistically significant(t=12.126,P＜0.001).②The high infiltration group of CD103+CD8+TRM cells had higher OS survival than the low infiltration group,and the difference was statistically significant(Log-Rank χ2=2.635,P<0.05).Conclusion The expression and infiltration of CD103+CD8+TRM cells in ESCC tissues were higher than those in adjacent tissues,and the patients with high infiltration had better immunotherapy efficacy and survival prognosis,which could be used as a new biological indicator to provide a new reference for clinical prediction of the efficacy and prognosis of ESCC immunotherapy.
