Exploring the Morphology of Calcium Hydroxide-Bone Cement Subcutaneously Induced Membrane and Osteogenic Factor Expression
10.11969/j.issn.1673-548X.2025.05.009
- VernacularTitle:氢氧化钙骨水泥皮下诱导膜形态及成骨因子表达情况
- Author:
Shaoxing LIANG
1
;
Zelong LIANG
;
Jiyuan ZHENG
Author Information
1. 570100 海口,海南医科大学急诊创伤学院
- Publication Type:Journal Article
- Keywords:
Calcium hydroxide-bone cement;
Calcium hydroxide;
Polymethyl methacrylate;
Induced membrane
- From:
Journal of Medical Research
2025;54(5):40-44
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate and observe the morphology of subcutaneous induced membrane and osteogenic factor expres-sion of the novel material calcium hydroxide-bone cement.Methods 15 4-week-old Specific Pathogen Free(SPF)female Kunming mice were randomly divided into three groups(n=5):experimental group,control group and blank group.A cut running deep under the skin was made on the back side,calcium hydroxide-bone cement was placed subcutaneously in the experimental group,the Polymethyl Methacrylate(PMMA)was placed subcutaneously in the control group,the blank group only with operation.After 6 weeks,the subcuta-neous tissue surrounded the material was taken from each group to be observed the pathological structure,calculated the collagen volume fraction(CVF)and detected the positive expression and relative expression of osteogenic factors such as bone morphogenetic protein-2(BMP-2),transforming growth factor-β1(TGF-β1)and vascular endothelial growth factor(VEGF)in the subcutaneous tissues.Results Comparing with the pathological sections of the blank group,there were induced membrane structure rich in collagenous fiber,microvessel and cell in subcutaneous tissue of the control and experimental groups.Comparing with control group,there were decrease of inflammatory cells,collagen content,microvessels,and disorder of collagenous fiber in experimental group.Comparing the CVF,average optical density(AOD)of osteogenic factors and grey scale value of protein bands in each group,the blank group,experimental group and control group increased sequentially,and there was a significant difference between each group(P＜0.05).Conclusion The new mate-rial,calcium hydroxide-bone cement,can form an induced membrane structure subcutaneously,but the 6-week induced membrane was not as good as the PMMA induced membrane during the same period in terms of morphology and osteogenic factor levels.
