Effect and mechanism of Yiqi tongfu xiere prescription on lipopoly-saccharide-induced acute lung injury in mice
10.12092/j.issn.1009-2501.2025.05.003
- VernacularTitle:益气通腑泻热方对脂多糖诱导的小鼠急性肺损伤的作用及机制研究
- Author:
Lijuan DU
1
;
Jianhua LIN
1
;
Jinghuan YE
1
;
Lu SONG
1
;
Yanfen PENG
1
;
Yuping LIU
1
Author Information
1. 深圳平乐骨伤科医院(深圳市坪山区中医院),深圳 518118,广东
- Publication Type:Journal Article
- Keywords:
Yiqi tongfu xiere prescription;
acute lung injury;
signal pathway;
cytokines;
mechanism of action
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2025;30(5):599-607
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigated the mechanism of action of Yiqi tongfu xiere prescription(YTX)in treating mice with lipopolysaccharide(LPS)-in-duced acute lung injury(ALI).METHODS:According to the random number table,24 C57BL/6 mice were divided into 4 groups:control group(Control),model group(LPS),low dose group(YTX-L)and high dose group(YTX-H).Except for the control group,the mice models of acute lung injury were established by intratracheal instillation of LPS solu-tion(5 mg/kg).The low and high dose treatment groups were given intragastric administration con-tinuously for 14 days.After 24 hours,the lung tis-sue,bronchoalveolar lavage fluid(BALF)and serum of the four groups were taken for follow-up detec-tion.The degree of pulmonary edema was evaluat-ed by wet weight coefficient(wet to dry ratio,W/D)of lung tissue.The degree of alveolar inflamma-tion and pulmonary fibrosis were evaluated by HE and Masson staining,and the contents of BALF and serum inflammatory cytokines IL-1β and IL-6 were detected by ELISA.The protein expressions of α-SMA,FN,Col-Ⅰ and Col-Ⅲ were measured by West-ern blot.Determination of α-SMA,FN,Col-Ⅰ,MAPK,NF-κB mRNA expression by RT-PCR method.RE-SULTS:Compared with LPS group,the contents of BALF,IL-1β and IL-6 in serum,Wmax D ratio,lung pathology,serum α-SMA,FN,Col-Ⅰ,Col-Ⅲ protein expression and α-SMA,FN,Col-Ⅰ,MAPK,NF-κB mRNA expression in treatment group were signifi-cantly lower than those in control group.CONCLU-SION:YTX can significantly reduce the levels of pul-monary fibrosis markers such as α-SMA,FN,Col-Ⅰand Col-Ⅲ by inhibiting the activation of MAPK/NF-κB signal pathway,and improve alveolar inflamma-tion and pulmonary fibrosis in mice with lung inju-ry,suggesting that YTX can treat acute lung injury and provide a theoretical basis for the clinical use of YTX.
