Application Evaluation of Nucleic Acid Detection of HPV in Cervical Secretions Based on Algal Glycolipid Nucleic Acid-free Extraction Technology
10.3969/j.issn.1671-7414.2025.02.036
- VernacularTitle:应用海藻糖脂核酸免提取技术对宫颈分泌物前处理进行HPV核酸检测的实验评价
- Author:
Mi ZHANG
1
;
Chunli YANG
1
;
Jun CHENG
1
;
Yuzhu DAI
1
Author Information
1. 中国人民解放军联勤保障部队第九〇三医院检验科,杭州 310013
- Publication Type:Journal Article
- Keywords:
HPV;
pre-treatment;
algal glycolipid nucleic acid-free extraction technology
- From:
Journal of Modern Laboratory Medicine
2025;40(2):191-194
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the application value of algal glycolipid nucleic acid-free extraction technology in human papillomaviruses(HPV)nucleic acid of cervical secretions.Methods From July 1 to September 30,2023,50 HPV samples were collected from the 903rd Hospital of PLA,and 12 quality control products of HPV nucleic acid were selected.Samples treated with algal glycolipid nucleic acid-free extraction technology were tested as the experimental group,samples treated with clinical routine detection kit were tested as the control group.The nucleic acid-free extraction technology for HPV detection was assessed for accuracy,sensitivity,precision and the correlation between the two methods.Results The HPV typing results of clinical samples(27 positive samples and 23 negative samples)and quality control products in the experimental group were consistent with those in the control group,with a 100%coincidence rate and high accuracy.The experimental group's detection limit reached 100 copies/ml,demonstrating great sensitivity.The intra-assay coefficient of variations were less than 5%,indicating good precision.The detection results(Ct values)of the two groups showed a strong correlation(P<0.05),and the linear regression equation was Y=2.524+0.901X,r=0.963.There was a good correlation between the Ct values of HPV nucleic acid testing between the experimental group and the control group.Conclusion Algal glycolipid nucleic acid-free extraction technology can be used in clinical cervical secretion HPV nucleic acid detection before treatment,which is suitable for clinical application and promotion.
