Cuttlebone extract on wound healing and VEGF/PI3K/Akt pathway in rats with refractory ulcers
10.12092/j.issn.1009-2501.2025.05.001
- VernacularTitle:海螵蛸提取物通过调控VEGF/PI3K/Akt通路对难愈性创面修复的影响及作用机制
- Author:
Guowei WANG
1
;
Tao ZHUO
;
Quanwei ZHENG
;
Mengying LI
;
Jiehui LI
;
Jianhang LIU
Author Information
1. 广西中医药大学,南宁 530000,广西
- Publication Type:Journal Article
- Keywords:
cuttlebone;
refractory wounds;
wound healing;
VEGF/PI3K/Akt pathway;
angiogen-esis
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2025;30(5):577-585
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the effect and mech-anism of cuttlebone extract regulating vascular en-dothelial growth factor(VEGF)/phosphatidylinosi-tol 3-kinase(PI3K)/protein kinase B(AKT)pathway on refractory wounds in rats.METHODS:Cuttle-bone extract(CE)was obtained by water extraction of cuttlebone.Fifty SD rats were randomly divided into negative Control group,Model group,Cuttle-bone extract low-dose(CE(L))group,Cuttlebone extract high-dose(CE(H))group,and cuttlebone ex-tract high-dose+inhibitor(CE(H)+LY294002)group.After the refractory wound model was successfully established,0.02％furacillin solution or cuttlebone extract solution were applied to the wound area of rats in each group,and the treatment was adminis-tered once a day.After 14 days of treatment for re-fractory wounds,the changes in wound healing,angiogenesis,inflammation and expression of relat-ed regulatory proteins were quantitatively ana-lyzed by measuring skin ulcer wound area,patho-logical sections,immunofluorescence staining,Eli-sa,Western blot,RT-qPCR and other methods.RE-SULTS:Compared with Model group,CE(L)and CE(H)groups can increase the number of epithelial cells and collagen,and promote the healing of re-fractory wound in rats.Serum VEGF,skin tissue mi-crovascular density,P-PI3K,P-AKT,VEGF protein ex-pression and mRNA expression levels of PI3K,Akt,VEGF and eNOS were increased(P＜0.05),while se-rum TNF-α and IL-6 levels were decreased(P＜0.05).LY294002 could partially reverse the repair-ing effect of high dose cuttlebone extract on refrac-tory wound(P＜0.05).CONCLUSION:Cuttlebone ex-tract can regulate the VEGF/PI3K/AKT signaling pathway,inhibit the inflammatory response of re-fractory wounds in rats,induce angiogenesis and promote wound healing.
